Fig 7.
From: Ribosome profiling reveals translational regulation of mammalian cells in response to hypoxic stress
uORFs regulated translation of the downstream main ORF. (a, b) Schematic representation of reporter constructs. a pEZX-GA02_uORF contained the human EPO, NAIP, PNPLA7 and SMPDL3B uORFs with the intact initiation (uAUG) and termination (UGA) codons cloned into the empty vector (pEZX-GA02). b In the pEZX-GA02_no-uORF construct, the uORF initiation codon was mutated (AUG → CUG; the cross represents the nonfunctional uORF). c, d, e, f EPO, NAIP, PNPLA7 and SMPDL3B uORFs and no-uORFs affected Gluc activity representing translational efficiency (relative Gluc activity/SEAP activity) under nomorxic and hypoxic conditions. Average values of at least three independent experiments are shown. The error bars indicate standard deviation. Statistical analysis was performed using Student’s t-test: * means p < 0.05 and ** means p < 0.01