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Fig. 6 | BMC Genomics

Fig. 6

From: Evaluation and comparison of computational tools for RNA-seq isoform quantification

Fig. 6

Significant difference in estimated read counts for transcript RPS28P7–001 resulting from STAR aligner. A total of 154 reads for RPS28P7–001 were simulated. a The estimated read counts from all eight methods are shown, and they are severely underestimated by the methods using STAR aligner. b The read coverage profiles (coloured in red) in RPS28P7–001 and RPS28–001. The peak paired-end read counts (both ends counted) are shown in brackets. Only a small fraction of reads were mapped back to the RPS28P7 region while the majority of reads were incorrectly mapped to the RPS28 gene

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