Table 1 Bacterial strains and plasmids used in this study

E. amylovora CFBP 1430

Wild type strain

[68]

E. amylovora T6-d1

ΔtssB-1/tssC-1T6SS cluster 1 deletion mutant, Cm^R

This study

E. amylovora T6-d3

ΔtssB-2/tssC-2 T6SS cluster 3 deletion mutant, Cm^R

This study

E. amylovora T6-d1d3

ΔtssB/tssC T6SS cluster 1 and 3 deletion mutant, Cm^R

This study

E. amylovora ΔhrpL

ΔhrpL Deletion mutant, Cm^R

[44]

E. coli DH5α

Φ80 lacZ M15, Δ(lacZYA-argF) U169, recA1, endA1,thi-1

Lab strain

pKD46

Red recombinase expressing plasmid, Ap^R

[38]

pKD3

Antibiotic resistance cassette template, Ap^R, Cm^R

[38]

pCP20

FLP encoding recombinase plasmid, Ap^R, Cm^R

[38]