Table 2 Summary table of selected significantly differentially expressed genes from the in vitro experiment comparing the WT to the T6SS double mutant
Name | Description | Locus tags | p value | Fold change |
|---|---|---|---|---|
Chemotaxis and motility | ||||
  cheW3   cheA3   motB3   motA3 | Chemotaxis protein CheW Chemotaxis protein CheA Chemotaxis protein MotB Flagellar motor protein MotA | EAMY_2650 EAMY_2651 EAMY_2652 EAMY_2653 | 1.64E-04 | 2.313 |
  cheB1   cheR1 | Chemotaxis response regulator protein-glutamate methylesterase Chemotaxis protein-glutamate O-methyltransferase | EAMY_2088 EAMY_2089 | 3.39E-05 | 3.227 |
  cheR3   cheB3 | Chemotaxis response regulator protein-glutamate methylesterase Chemotaxis protein-glutamate O-methyltransferase | EAMY_2696 EAMY_2697 | 2.61E-07 | 8.574 |
  cheY1 | Response regulator | EAMY_2087 | 1.12E-03 | 3.681 |
  flgA1   flgB1   flgC1   flgG1   flgH1   flgI1   flgJ1 | Flagellar basal body P-ring biosynthesis protein FlgA Flagellar basal body rod protein FlgB Flagellar basal body rod protein FlgC Flagellar basal body rod protein FlgG Flagellar basal body L-ring protein Flagellar basal body P-ring protein Flagellar rod assembly protein/muramidase FlgJ | EAMY_1452 EAMY_1453 EAMY_1454 EAMY_1458 EAMY_1459 EAMY_1460 EAMY_1461 | 1.32E-03 1.88E-05 4.89E-04 6.72E-04 3.03E-05 3.14E-03 | 3.580 6.364 4.028 3.891 5.502 2.497 |
  flgM   flhB3   flhA3   flhE3 | Anti-sigma-28 factor FlgM Flagellar biosynthesis protein FlhB Flagellar biosynthesis protein FlhA Flagellar biosynthesis protein FlhE | EAMY_1451 EAMY_2700 EAMY_2701 EAMY_2702 | 3.56E-04 3.00E-07 | −4.084 3.655 |
  flhC1   flhD1 | Transcriptional activator FlhC Flagellar transcriptional activator FlhD | EAMY_2099 EAMY_2100 | 2.81E-04 | 3.272 |
  flhD3 | Transcriptional activator FlhD | EAMY_2655 | 4.84E-06 | −7.945 |
  fliD3 | Flagellar hook protein FliD | EAMY_2674 | 1.58E-03 | 4.408 |
  fliE1 | Flagellar hook-basal body complex protein FliE | EAMY_1512 | 7.67E-11 | 20.252 |
  fliI1   fliH1   fliG1   fliF1 | Flagellum-specific ATP synthase FliI Flagellar assembly protein FliH Flagellar motor switch protein FliG Flagellar M-ring protein FliF | EAMY_1508 EAMY_1509 EAMY_1510 EAMY_1511 | 4.54E-04 | 5.205 |
  fliL1 | Flagellar basal body-associated protein FliL | EAMY_1505 | 2.80E-03 | 3.630 |
  fliP1   fliO1 | Flagellar biosynthetic protein FliP Flagellar biosynthetic protein FliO | EAMY_1501 EAMY_1502 | 1.03E-05 | 4.532 |
  fliR3   fliQ3 | Flagellar biosynthetic protein FliR Flagellar export apparatus protein FliQ | EAMY_2682 EAMY_2683 | 6.16E-12 | −11.876 |
  fliS1 | Flagellar protein FliS | EAMY_2143 | 6.07E-04 | 3.945 |
  fliT1 | Flagellar biosynthesis protein FliT | EAMY_2144 | 7.21E-04 | 3.918 |
  fliZ | Flagellar regulatory protein FliZ | EAMY_2138 | 2.41E-12 | 32.447 |
Iron acquisition | ||||
  dfoJ | Cytochrome c biogenesis protein CcmH | EAMY_3238 | 1.60E-05 | -6.869 |
Type III secretion systems | ||||
  hopPtoC | Cysteine protease | EAMY_0744 | 8.19E-06 | -6.589 |
  hrpF   hrpG   hrcC | HPr kinase Hypothetical protein EscC/YscC/HrcC family type III secretion system outer membrane ring protein | EAMY_0547 EAMY_0548 EAMY_0549 | 2.05E-04 | −2.888 |
  hrcJ   hrpD   hrpE | EscJ/YscJ/HrcJ family type III secretion inner membrane ring protein Hypothetical protein Type III secretion system protein | EAMY_0544 EAMY_0545 EAMY_0546 | 4.80E-05 | −2.809 |
  hrpA1 | Hrp pili protein HrpA | EAMY_0542 | 4.74E-03 | −4.500 |
  hrpK | Pathogenicity locus protein hrpK | EAMY_0519 | 6.02E-05 | 6.821 |
  hsvA | Hypothetical protein | EAMY_0520 | 1.99E-03 | −3.706 |
  invA3   invB | Type III secretion system protein InvA Hypothetical protein | EAMY_1580 EAMY_1581 | 8.99E-06 | 4.028 |
  spaT1   sipB1 | CesD/SycD/LcrH family type III secretion system chaperone Type III secretion system protein | EAMY_0789 EAMY_0790 | 4.20E-07 | −4.408 |
  spaI1   spaM1   spaN1   spaO1   spaP1   spaQ1 | ATP synthase SpaL Hypothetical protein Hypothetical protein Type III secretion system protein EscR/YscR/HrcR family type III secretion system export apparatus protein Type III secretion system protein SpaQ | EAMY_0781 EAMY_0782 EAMY_0782 EAMY_0784 EAMY_0785 EAMY_0786 | 8.13E-06 | −3.074 |
  spaK | Hypothetical protein | EAMY_0780 | 4.73E-04 | 7.835 |
  spaS3 | EscU/YscU/HrcU family type III secretion system export apparatus switch protein | EAMY_1589 | 6.35E-04 | 4.287 |