Fig. 2

Comparative transcriptome analysis. a Alignment and mapping statistics, Y-axis representing the total number of reads sequenced from each library. More reads were captured in the RD as compared to the PA enriched libraries. b Bio-detection plot, Y-axis representing the percentage of genomic features. The bars correspond to the percentage of each biotype in the human reference genome (Ensembl 80). c The percentage of mapped reads to intragenic, intronic and exonic regions, Y-axis shows the read mapping rates. The replicate samples with the RD library showed much higher variation in the number of exonic reads compared to samples from different libraries. d Y-axis on the left and Y-axis on the right represent the number and rates of rRNA mapped reads, respectively. The RD method is more effective at rRNA removal as compared to the PA protocol. e The heatmap represents the patient, disease and library specific variations. Genes with log2 RPKM >2 and CV >20 were selected for hierarchical clustering. Clustering was performed with both genes and samples using a Euclidean distance and complete linkage method