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Table 2 Microarray data validation by RT-PCR. This table shows a comparison between the differential expression detected by microarray with the corresponding transcript values using RT-PCR. Using RT-PCR, we validated 20/24 genes suggesting 83% concordance between the two methods

From: Expression profiling of genes regulated by sphingosine kinase1 signaling in a murine model of hyperoxia induced neonatal bronchopulmonary dysplasia

  

WT RA

Fold change

WT HO

Fold change

Sphk1 −/− RA

Fold change

Sphk1 −/− HO

Fold change

Gadd45a

Microarray Fold Change

1

3.63168

0.921811

1.002221

RTPCR

1

2.579674

0.908044

0.967095

Gadd45g

Microarray Fold Change

1

8.38319

1.43496

2.705904

RTPCR

1

6.245498

1.100627

2.153558

Cdkn1a

Microarray Fold Change

1

7.99461

1.2607

7.897151

RTPCR

1

13.72415

1.537082

12.61852

Bax

Microarray Fold Change

1

3.87613

1.16573

2.869782

RTPCR

1

3.43179

1.032757

2.079701

Serpine1

Microarray Fold Change

1

15.4903

1.32755

5.559448

RTPCR

1

8.024327

0.94901

3.421921

Cdkn1b

Microarray Fold Change

1

0.708453

1.16238

0.75834

RTPCR

1

0.740789

1.324503

1.318684

Serpina3n

Microarray Fold Change

1

75.388

3.72853

14.3611

RTPCR

1

18.49097

1.648514

4.635865

Saa3

Microarray Fold Change

1

5.19527

0.517552

15.89733

RTPCR

1

4.095082

0.74759

11.46964

Bmp7

Microarray Fold Change

1

0.907484

0.817163

1.759001

RTPCR

1

0.821678

0.857167

3.075663

Wnt5a

Microarray Fold Change

1

0.2359

1.07608

0.638945

RTPCR

1

0.152372

0.926221

0.623315

Cdh2

Microarray Fold Change

1

0.642012

0.879343

0.764438

RTPCR

1

0.199199

0.924477

0.879041

Aplnr

Microarray Fold Change

1

0.049004

1.06134

0.147246

RTPCR

1

0.092198

1.04678

0.375555

S1pr1

Microarray Fold Change

1

2.26942

1.17521

1.116978

RTPCR

1

4.894727

0.889052

0.87604

S1pr2

Microarray Fold Change

1

0.921563

0.750461

0.997753

RTPCR

1

3.656166

0.81154

1.212284

S1pr3

Microarray Fold Change

1

0.933923

0.936387

1.03291

RTPCR

1

0.415816

0.944109

0.978297

Lox

Microarray Fold Change

1

1.30887

1.04026

1.181007

RTPCR

1

2.450447

0.932973

1.175259

Il6st

Microarray Fold Change

1

1.79941

1.29187

1.072552

RTPCR

1

2.79062

0.905679

0.820019

Il6

Microarray Fold Change

1

5.55994

1.29187

3.028182

RTPCR

1

6.072546

0.791731

1.45494

Il6ra

Microarray Fold Change

1

1.64621

1.40755

1.136742

RTPCR

1

2.101202

1.082853

0.876843

Sgpl1

Microarray Fold Change

1

0.997442

0.890375

0.938402

RTPCR

1

0.424413

0.963454

0.875726

Sphk1

Microarray Fold Change

1

3.70231

1.51325

1.556574

RTPCR

1

4.364135

1.047004

1.149245

Lpar3

Microarray Fold Change

1

1.78

0.95

0.83

RTPCR

1

2.153558

1.008994

0.773786

Chi3l3

Microarray Fold Change

1

26.0615

4.07383

1.2288

RTPCR

1

22.807

3.806

1.5576

Stfa1

Microarray Fold Change

1

0.0663964

0.505696

1.89924

RTPCR

1

0.0537

.62

2.086

  1. Bold Differentially regulated gene expression