Maternal nutrient restriction in mid-to-late gestation influences fetal mRNA expression in muscle tissues in beef cattle

Paradis, Francois; Wood, Katie M.; Swanson, Kendall C.; Miller, Stephen P.; McBride, Brian W.; Fitzsimmons, Carolyn

doi:10.1186/s12864-017-4051-5

Table 1 Primer sequences and amplification conditions for gene expression measured by real-time PCR

From: Maternal nutrient restriction in mid-to-late gestation influences fetal mRNA expression in muscle tissues in beef cattle

Gene	GenBank Accession #	Primer	Sequence 5′ - 3’	Product size (bp)
ACTB	NM_173979.3	Fwd	CTCTTCCAGCCTTCCTTCCT	245
ACTB	NM_173979.3	Rev	CCAATCCACACGGAGTACTTG	245
EEF1A2	NM_001037464.1	Fwd	AGTTCACGTCCCAGGTCATC	149
EEF1A2	NM_001037464.1	Rev	CTCCAACTTCTTGCCAGAGC	149
FLT1	NM_001191132.2	Fwd	AGGGAAGAAGGTGGTCATCC	185
FLT1	NM_001191132.2	Rev	TGACTGTTGTCTCGCAGGTC	185
GAPDH	NM_001034034.1	Fwd	TGACCCCTTCATTGACCTTC	143
GAPDH	NM_001034034.1	Rev	GATCTCGCTCCTGGAAGATG	143
HMBS	NM_001046207.1	Fwd	CTACTTCGCTGCATTGCTGA	105
HMBS	NM_001046207.1	Rev	CAGGTACAGTTGCCCATCCT	105
IGF1	NM_001077828	Fwd	GATGCTCTCCAGTTCGTGTG	141
IGF1	NM_001077828	Rev	CTCCAGCCTCCTCAGATCAC	141
IGF1R	NM_001244612	Fwd	CAAAGGCAATCTGCTCATCA	139
IGF1R	NM_001244612	Rev	CAGGAAGGACAAGGAGACCA	139
IGF2	NM_174087.3	Fwd	CCAGCGATTAGAAGTGAGCC	95
IGF2	NM_174087.3	Rev	AGACCTAGTGGGGCGGTC	95
IGF2R ^a	NM_174352	Fwd	GCAATGCTAAGCTTTCGTATTACG	188
IGF2R ^a	NM_174352	Rev	GGTGTACCACCGGAAGTTGTATG	188
INSR	XM_002688832	Fwd	CCTATGCCCTGGTGTCACTT	114
INSR	XM_002688832	Rev	GCTGCCTTAGGTTCTGGTTG	114
KDR	NM_001110000.1	Fwd	ATCGAAGTTTCCTGCACAGC	133
KDR	NM_001110000.1	Rev	TCACCCTGCGGATAGTTAGG	133
MEF2A	NM_001083638	Fwd	CAATGCCAACTGCCTACAAC	130
MEF2A	NM_001083638	Rev	TGTCCTAAATGGTGCTGCTG	130
MYOD1	NM_001040478	Fwd	GAACACTACAGCGGCGACTC	121
MYOD1	NM_001040478	Rev	AGTAAGTGCGGTCGTAGCAG	121
MYOG	NM_001111325	Fwd	CAGTGAATGCAGCTCCCATA	164
MYOG	NM_001111325	Rev	CGACATCCTCCACTGTGATG	164
PGF	NM_173950	Fwd	GGAACATTTCATCGGAGGTG	136
PGF	NM_173950	Rev	CAGGATGGGCTGAATAGATG	136
PPARG	NM_181024,2	Fwd	ACCACCGTTGACTTCTCCAG	137
PPARG	NM_181024,2	Rev	ACAGGCTCCACTTTGATTGC	137
PPIA	NM_178320.2	Fwd	GTCAACCCCACCGTGTTCT	132
PPIA	NM_178320.2	Rev	TCCTTTCTCTCCAGTGCTCAG	132
SLC2A1	NM_174602.2	Fwd	ACACAGCCTTCACTGTCGTG	156
SLC2A1	NM_174602.2	Rev	TGCTCAGGTAGGACATCCAG	156
SLC2A3	NM_174603	Fwd	CCTCTGATCTTCGCCATCTC	170
SLC2A3	NM_174603	Rev	AAGGACCACAGGGATGTGAG	170
SLC7A1	NM_001135792.1	Fwd	AGCAGCAGCGATTCTCAGAC	158
SLC7A1	NM_001135792.1	Rev	AGGCAGAAGGTGATGACCAG	158
SRF	NM_001206016	Fwd	CGGCTTTGAAGAGACAGACC	101
SRF	NM_001206016	Rev	GCAGGTTGGTGACGGTAAAC	101
VEGFA	NM_174216.1	Fwd	AGACCCTGGTGGACATCTTC	185
VEGFA	NM_174216.1	Rev	TATGTGCTGGCTTTGGTGAG	185
YWHAZ	NM_174814.2	Fwd	AGACGGAAGGTGCTGAGAAA	123
YWHAZ	NM_174814.2	Rev	CGTTGGGGATCAAGAACTTT	123

^aSpicer et al. [48]

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ISSN: 1471-2164

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