Maternal nutrient restriction in mid-to-late gestation influences fetal mRNA expression in muscle tissues in beef cattle

Table 2 Primers used to amplify each of the three DMRs investigated

Gene	Location	Primer	Sequence 5′ - 3’	Product size (bp)	Total CpG sites (Analysable site)
IGF2R DMR2	UCSC_Btau_4.6.1 Chr9:100,467,361–100,467,716	Fwd	aggaagagagGAGTAAAGATTTGAAATTGAAAGTAG	356	17 (11)
IGF2R DMR2	UCSC_Btau_4.6.1 Chr9:100,467,361–100,467,716	Rev	cagtaatacgactcactatagggagaaggctAACCTTCTCAACACCTTACTCAAAA	356	17 (11)
IGF2 DMR2	UCSC_Btau_4.6.1 Chr29:51,079,954–51,080,478	Fwd	aggaagagagGTATTTTGGGTATTTGGGGTAGTTT	525	22 (10)
IGF2 DMR2	UCSC_Btau_4.6.1 Chr29:51,079,954–51,080,478	Rev	cagtaatacgactcactatagggagaaggctATTCTAATCCCCTCAACCAAATAAA	525	22 (10)
IGF2/H19 ICR	UCSC_Btau_4.6.1 Chr29:51,165,094–51,165,593	Fwd	aggaagagagGGATAGGAGATTAGGTTTAGAGGGG	500	17 (14)
IGF2/H19 ICR	UCSC_Btau_4.6.1 Chr29:51,165,094–51,165,593	Rev	cagtaatacgactcactatagggagaaggctAAAAAAAACTATAAAATCCTCCTACCC	500	17 (14)

ISSN: 1471-2164