Table 4 Impact of maternal nutrition during mid-to-late gestation on the methylation status of IGF2 DMR2 as assessed by Sequenom Epityper analysis

IGF2 DMR2 Chr29:51,079,954–51,080,478

1, 2, 3

29, 38, 47

29.1 ± 1.4

27.2 ± 0.9

0.38

R = −0.40, p = 0.066

10,11

173, 183

52.3 ± 1.6

48.6 ± 1.3

0.11

R = −0.67, P = 0.0007

12, 13, 14

189, 192, 198

40.4 ± 1.5

35.7 ± 0.9

0.019

R = −0.70, P = 0.0003

15, 16

202, 206

45.8 ± 1.4

41.9 ± 1.0

0.07

R = −0.70, P = 0.0003

20

257

68.1 ± 1.5

63.3 ± 1.4

0.039

R = −0.73, P = 0.0001

21, 22

287, 293

48.9 ± 1.4

47.5 ± 0.6

0.44

R = −0.57, P = 0.0059

28

384

67.9 ± 2.4

63.0 ± 3.3

0.13

R = −0.25, P = 0.26

29

390

89.1 ± 1.8

82.5 ± 1.3

0.014

R = −0.64, P = 0.0013

34

482

76.7 ± 4.4

71.6 ± 2.4

0.31

R = −0.25, P = 0.29

35

493

68.4 ± 1.0

65.9 ± 0.7

0.073

R = −0.71, P = 0.0002

All

na

51.3 ± 1.3

47.4 ± 0.9

0.025

R = −0.65, P = 0.001

IGF2 DMR2 Chr29:51,079,954–51,080,478

1, 2, 3

29, 38, 47

31.3 ± 0.8

29.9 ± 0.7

0.28

R = −0.66, P = 0.0008

10,11

173, 183

55.0 ± 1.2

51.6 ± 1.0

0.042

R = −0.68, P = 0.0005

12, 13, 14

189, 192, 198

41.8 ± 1.3

38.1 ± 0.8

0.06

R = −0.78, P ≤ 0.0001

15, 16

202, 206

46.3 ± 0.8

43.7 ± 0.8

0.056

R = −0.74, P ≤ 0.0001

20

257

69.4 ± 1.3

66.4 ± 1.1

0.16

R = −0.70, P = 0.0003

21, 22

287, 293

50.3 ± 1.2

47.3 ± 1.2

0.11

R = −0.49, P = 0.019

28

384

71.7 ± 3.7

61.6 ± 1.9

0.075

R = −0.63, P = 0.0017

29

390

87.8 ± 1.5

85.8 ± 1.8

0.46

R = −0.44, P = 0.038

34

482

80.1 ± 3.6

75.2 ± 4.1

0.53

R = −0.56, P = 0.011

35

493

68.5 ± 1.0

66.6 ± 0.7

0.12

R = −0.69, P = 0.0004

All

na

52.8 ± 1.1

49.6 ± 0.9

0.086

R = −0.79, P ≤ 0.0001