Table 1 List of genes and TFs validated through qRT-PCR
S. no. | Gene_ID | Fold change | Regulation | Stage | GenBank ID | Closest Arabidopsis homolog | Description | Forward Primer | Reverse Primer |
|---|---|---|---|---|---|---|---|---|---|
Gene 1 | GhiAffx.7054.1.S1_at | 16.50139 | Up | 0 dpa | DW509967.1 | AT5G59550.1 | zinc finger (C3HC4-type RING finger) family protein | ACTTCCACCATGAGACCTCAAGCA | ATTCGGGTCATGGAGATGTGACGA |
Gene 2 | GhiAffx.2527.1.S1_s_at | 151.18683 | Down | 0 dpa | DW497370.1 | AT5G53120.6 | spermidine synthase 3 | CAACATGCAGGTGAAATCGGAACC | CAAGCAAACACATTGCTGCGTCAC |
Gene 3 | Ghi.8931.1.S1_a_at | 87.72372 | Down | 0 dpa | DT457712 | AT4G27520.1 | unknown | TTCAGCTAAGCCCGTTTACACCCT | TGAAACAACGGGAGTTGAAGAGGC |
Gene 4 | Ghi.7853.1.S1_at | 5.8283057 | Down | 0 dpa | AF443118.1 | AT1G01630.1 | Sec14p-like phosphatidylinositol transfer family protein | TCCGCTAAAGCGAGGGAAGAAGAA | GGAACATGCTCGATGCTTTCTCCA |
Gene 5 | Ghi.7950.1.S1_at | 81.1664 | Down | 10 dpa | AY366083.1 | AT5G06720.1 | peroxidase 2 | CGCTTTGTCCGGTGTTCACACATT | TGTTTGCATATGTCGCGTTGAGGG |
Gene 6 | GhiAffx.46297.1.S1_s_at | 58.77188 | Down | 10 dpa | AI054544 | AT4G17030.1 | expansin-like B1 | ATCCGTCGATATTTGGCAGGAGGA | TCAACTGATGTTCTCCCGATGGCA |
Gene 7 | Ghi.3370.1.A1_at | 45.29446 | Down | 10 dpa | DT463939 | AT1G33590.1 | Leucine-rich repeat (LRR) family protein | AGGCGATAATGGAGGTCCACACAA | CCTGCGGCGATTAATGGGTTCAAT |
Gene 8 | Ghi.8023.1.S1_at | 39.720764 | Down | 10 dpa | DQ116443.1 | AT1G12010.1 | 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein | ACCAAAGTCAGCAACTACCCTCCA | TTGAGGAGTTGAAGCCCACTGACT |
Gene 10 | GhiAffx.19125.1.A1_at | 19.172623 | Down | 10 dpa | DW487672.1 | AT1G12780.1 | UDP-D-glucose/UDP-D-galactose 4-epimerase 1 | AGGTTGGCCATAGACTGTTGCAGA | AAAGTGTCGGTAACCCTCGTCGTT |
Gene 11 | Ghi.6496.1.S1_a_at | 18.636307 | Down | 10 dpa | CD486227 | AT4G02380.1 | senescence-associated gene 21 | AGAGAAGGTTTCATGGGTTCCCGA | ACTTCTTCAAGAGGGTGGCTCTGA |
Gene 12 | Ghi.1552.1.S1_s_at | 18.200262 | Down | 10 dpa | DN779868 | AT1G47960.1 | cell wall/vacuolar inhibitor of fructosidase 1 | GCAGATCACAGCGCTGCTTAAAGT | TCGCCTTTCGTGACAGCTTCTACA |
Gene 13 | Ghi.8448.1.S1_x_at | 11.607274 | Down | 10 dpa | AF521240.1 | AT5G12250.1 | beta-6 tubulin | Â | Â |
Gene 14 | Ghi.6088.2.A1_s_at | 10.754808 | Down | 10 dpa | DV849489 | AT3G45640.1 | mitogen-activated protein kinase 3 | TGAGCAGCAACCATTGGGAGAAGA | ACTTGGACTGGCGCACTTAGAAGA |
Gene 15 | Ghi.5521.1.A1_s_at | 8.330377 | Down | 10 dpa | DT047436 | AT5G01210.1 | HXXXD-type acyl-transferase family protein | TCAATAGCTGCCATAGTTTCCGGC | TTGAAGTTGGCAGTTAGGAGTGGC |
Gene 16 | Gra.2314.1.S1_at | 6.476485 | Down | 10 dpa | CO126415 | AT3G13750.1 | beta galactosidase 1 | CGAGAGGCAATGAACCGTTAGCTT | GGCATAATCACAAGCACCGCAACT |