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Fig. 2 | BMC Genomics

Fig. 2

From: Variation and evolution of polyadenylation profiles in sauropsid mitochondrial mRNAs as deduced from the high-throughput RNA sequencing

Fig. 2

Characterization of Takydromus tachydromoides mt-mRNAs using RNA-Seq reads. a Gene organization of T. tachydromoides mtDNA with major polyadenylation sites. Major polyadenylation sites for H-strand-transcribed and L-strand-transcribed RNAs supported by 10 or more independent (forward and reverse paired-end reads derived from a common cDNA fragment were merged for the counting) polyA-containing reads are shown over and under the column, respectively, with the number of the supporting polyA-containing reads. Note that no major polyadenylation sites were found for L-strand transcripts of T. tachydromoides. Changes in mRNA polyadenylation sites as compared to the human [7] are highlighted by a circle. Refer to the legend of Fig. 1 for gene abbreviations and other details. b Mapping of RNA-Seq reads to T. tachydromoides mtDNA. At the bottom, frequency of the RNA-Seq reads covering each site of the 17,923-bp mtDNA sequence is plotted in the vertical direction. In the horizontal direction, ranges for protein (from a start codon to a stop codon) and rRNA genes are shown along with tRNA gene cluster regions (IQM, WANCY and HSL). A sharp decline of read frequencies, which corresponds to the new polyadenylation site for ND5 mRNA (see text), is indicated by a red arrow. At the top, the slope of the mapped read frequencies as measured by sliding a 10-nucleotide window is plotted in the vertical direction. Ranges for H-strand-transcribed mRNAs estimated are illustrated by horizontal arrows

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