Fig. 2From: Transcriptome analysis reveals differences in mechanisms regulating cessation of luteal function in pregnant and non-pregnant dogsRepresentative heatmaps showing the RNA-Seq analysis of differentially expressed genes (DEG) of two contrasts: a “mid-gestation over antigestagen”, and b “luteolysis over antigestagen”, are presented. For each gene the red color indicates high expression relative to the average expression of the gene, while the blue color indicates low expression. a 492 genes were found for the contrast “mid-gestation over antigestagen” based on the p < 0.01 threshold (shown). Of these, 92 DEG passed the FDR < 0.1 selection (adjusted p-value), of which 38 genes were more and 54 genes were less expressed at mid-gestation. These genes were used for further downstream analyses. b 1480 DEG were identified in the contrast “luteolysis over antigestagen”, 793 were more and 687 were less expressed in the antigestagen group compared to the luteolysis group (p < 0.01, FDR < 0.1). The main functional terms overrepresented in each of the groups are listed (details, including statistics are presented in the text). The entire list of DEG identified genes is provided as Additional files 3 and 4 Back to article page