Fig. 2

Gene expression in Maf-S knockdowns relative to GFP injected controls. qPCR of three transcripts regulated by the cnc-Maf-S pathway in Drosophila (Gnmt, Jheh1, Jheh2) and five selected from the Maf-S co correlated genes in Anopheles (CYP4H17, GSTD3, ABCA3, CYP6M2, GSTD1) was performed on Maf-S knockdown cDNA. Data are shown normalised against expression in GFP-injected controls. Significance (p ≤ 0.05), as determined by a Welch’s t-test, is indicated by an *. Each data point represents the mean of three biological replicates each comprising cDNA from 7 to 10 females, 72 h post injection (actual age of mosquitoes = 7–8 days)