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Fig. 1 | BMC Genomics

Fig. 1

From: Lack of 14-3-3 proteins in Saccharomyces cerevisiae results in cell-to-cell heterogeneity in the expression of Pho4-regulated genes SPL2 and PHO84

Fig. 1

Heterogenic expression of SPL2-GFP in bmh1∆ cells. a left panel, flow cytometry of BY4741 cells (line, no fill), BY4741 SPL2-GFP cells (red) and bmh1Δ SPL2-GFP cells (blue) grown in YNB medium with 50 mM KCl; right panel, flow cytometry of BY4741 cells (line, no fill), bmh2Δ SPL2-GFP cells (red) and bmh1∆ SPL2-GFP cells (blue) grown in YNB medium with 50 mM KCl. b confocal microscopy of BY4741 SPL2-GFP, bmh1Δ SPL2-GFP and bmh2Δ SPL2-GFP cells grown in YNB medium with 50 mM KCl. Scale bar 10 μm. c confocal microscopy of BY4741 SPL2-GFP, bmh1Δ SPL2-GFP and bmh2Δ SPL2-GFP cells grown for 2 h in YNB medium without KCl. d confocal microscopy of BY4741 SPL2-GFP, bmh1Δ SPL2-GFP and bmh2Δ SPL2-GFP cells grown for 2 h in YNB medium without phosphate. e left panel: flow cytometry of BY4741 cells (line, no fill), BY4741 SPL2-GFP cells grown in YNB medium with 50 mM KCl (blue) or without KCl (red). Right panel: flow cytometry of BY4741 cells (line, no fill), bmh1Δ SPL2-GFP cells grown in YNB medium with 50 mM KCl (blue) or without KCl (red). f left panel: flow cytometry of BY4741 cells (line, no fill), BY4741 SPL2-GFP cells containing YCplac33 (blue) and BY4741 SPL2-GFP cells containing YCplac33[BMH1] (red) grown in YNB medium with 50 mM KCl. Right panel flow cytometry of BY4741 cells (line, no fill), bmh1Δ SPL2-GFP cells containing YCplac33 (blue) and bmh1Δ SPL2-GFP cells containing YCplac33[BMH1](red) grown in YNB medium with 50 mM KCl

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