Fig. 2
Effect of bmh1 deletion on the PHO84 promoter and activation by low potassium or phosphate. a confocal microscopy of wild type (BY4741) cells expressing GFP under control of the PHO84 promoter (BY4741 (PPHO84-GFP)) after cultivation in YNB medium with 50 mM KCl, after cultivation for 1 h in YNB medium lacking potassium (0 mM KCl) and after cultivation for 1 h in YNB medium lacking phosphate (0 mM phosphate). b confocal microscopy of ∆bmh1 cells expressing GFP under control of the PHO84 promoter (∆bmh1 (PPHO84-GFP)) after cultivation in YNB medium with 50 mM KCl, after cultivation for 1 h in YNB medium lacking potassium (0 mM KCl) and after cultivation for 1 h in YNB medium lacking phosphate (0 mM phosphate). c confocal microscopy of wild type (BY4741) cells expressing GFP under control of the CYC1 promoter (BY4741 (Pcyc1-GFP)) after cultivation in YNB medium with 50 mM KCl, after cultivation for 1 h in YNB medium lacking potassium (0 mM KCl) and after cultivation for 1 h in YNB medium lacking phosphate (0 mM phosphate). d confocal microscopy of ∆bmh1 cells expressing GFP under control of the CYC1 promoter (∆bmh1 (PCYC1-GFP)) after cultivation in YNB medium with 50 mM KCl, after cultivation for 1 h in YNB medium lacking potassium (0 mM KCl) and after cultivation for 1 h in YNB medium lacking phosphate (0 mM phosphate)