Fig. 3

Effect of an additional copy of PHO4 on SPL2-GFP expression. a Confocal microscopy of BY4741 SPL2-GFP and ∆bmh1 SPL2-GFP with integrated pRS305 or pRS305[PHO4] cells grown in YNB medium with 50 mM KCl. Scale bar 10 μm. b Flow cytometry of BY4741 SPL2-GFP (left panel) and ∆bmh1 SPL2-GFP (right panel) with integrated pRS305 (blue) or pRS305[PHO4] (red) cells grown in YNB medium with 50 mM KCl. Line, no fill: BY4741 cells