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Table 2 Plasmids

From: Lack of 14-3-3 proteins in Saccharomyces cerevisiae results in cell-to-cell heterogeneity in the expression of Pho4-regulated genes SPL2 and PHO84

Plasmid Properties Source/Reference
pRS316 Yeast centromeric plasmid. URA3 marker. [52]
pRS316[PPHO84-GFP-TPHO84] (pRUL1336) pRS316 containing the PHO84 promoter (600 bp), GFP and the PHO84 terminator (436 bp) This study
pRS316[PCYC1-GFP-TCYC1] (pRUL1339) pRS316 containing the CYC1 promoter (300 bp), GFP and the CYC1 terminator (220 bp) This study
pRS316[PSPL2-GFP-TSPL2] (pRUL1354) pRS316 containing the SPL2 promoter (647 bp), GFP and the SPL2 terminator (324 bp) This study
pUG6 Plasmid containing the KAN.MX casette for gene disruptions [53]
pUG36 Centromeric plasmid to make N-terminal GFP fusions. Güldener and Hegemann, unpublished
pRS305 Yeast integration plasmid. LEU2 marker. [52]
pRS305[PPHO84-GFP-TPHO84] (pRUL1359) pRS305 containing the PHO84 promoter (600 bp), GFP and the PHO84 terminator (436 bp) This study
pRS305[PCYC1-GFP-TCYC1] (pRUL1360) pRS305 containing the CYC1 promoter (300 bp), GFP and the CYC1 terminator (220 bp) This study
pRS313 Yeast centromeric plasmid. HIS3 selection marker. [52]
pRS313[PHO4](pRUL1334) pRS313 containing PHO4 [7]
pRS313[BMH1] (pRUL1330) pRS313 containing a 3.2 kb genomic DNA fragment with BMH1 I.G. Anemaet, unpublished results
pRS305[PHO4] (pRUL1358) pRS305 containing PHO4 This study
YCplac33 Yeast centromeric plasmid. URA3 marker [54]
YCplac33[BMH1] YCplac33 with a 3.2 kb genomic DNA fragment with BMH1 [55]