Lack of 14-3-3 proteins in Saccharomyces cerevisiae results in cell-to-cell heterogeneity in the expression of Pho4-regulated genes SPL2 and PHO84

Table 2 Plasmids

Plasmid	Properties	Source/Reference
pRS316	Yeast centromeric plasmid. URA3 marker.	[52]
pRS316[P_PHO84-GFP-T_PHO84] (pRUL1336)	pRS316 containing the PHO84 promoter (600 bp), GFP and the PHO84 terminator (436 bp)	This study
pRS316[P_CYC1-GFP-T_CYC1] (pRUL1339)	pRS316 containing the CYC1 promoter (300 bp), GFP and the CYC1 terminator (220 bp)	This study
pRS316[P_SPL2-GFP-T_SPL2] (pRUL1354)	pRS316 containing the SPL2 promoter (647 bp), GFP and the SPL2 terminator (324 bp)	This study
pUG6	Plasmid containing the KAN.MX casette for gene disruptions	[53]
pUG36	Centromeric plasmid to make N-terminal GFP fusions.	Güldener and Hegemann, unpublished
pRS305	Yeast integration plasmid. LEU2 marker.	[52]
pRS305[P_PHO84-GFP-T_PHO84] (pRUL1359)	pRS305 containing the PHO84 promoter (600 bp), GFP and the PHO84 terminator (436 bp)	This study
pRS305[P_CYC1-GFP-T_CYC1] (pRUL1360)	pRS305 containing the CYC1 promoter (300 bp), GFP and the CYC1 terminator (220 bp)	This study
pRS313	Yeast centromeric plasmid. HIS3 selection marker.	[52]
pRS313[PHO4](pRUL1334)	pRS313 containing PHO4	[7]
pRS313[BMH1] (pRUL1330)	pRS313 containing a 3.2 kb genomic DNA fragment with BMH1	I.G. Anemaet, unpublished results
pRS305[PHO4] (pRUL1358)	pRS305 containing PHO4	This study
YCplac33	Yeast centromeric plasmid. URA3 marker	[54]
YCplac33[BMH1]	YCplac33 with a 3.2 kb genomic DNA fragment with BMH1	[55]

ISSN: 1471-2164