Lack of 14-3-3 proteins in Saccharomyces cerevisiae results in cell-to-cell heterogeneity in the expression of Pho4-regulated genes SPL2 and PHO84

Table 5 RNA levels of PHO84 and SPL2 determined by qRT-PCR and complementation by wild type BMH1

	RNA level (arbitrary units) (± SD; n = 3)
Strain	PHO84		SPL2
Strain	50 mM	0 mM	50 mM	0 mM
BY4741 pRS313^a	0.18 ± 0.03	1.10 ± 0.20	0.24 ± 0.12	1.50 ± 0.34
bmh1Δ pRS313	0.03 ± 0.01^b	0.98 ± 0.31	0.05 ± 0.02	1.44 ± 0.21
BY4741 pRS313[BMH1]	1.01 ± 0.01	1.23 ± 0.40	1.05 ± 0.19	2.03 ± 0.22
bmh1Δ pRS313[BMH1]	0.18 ± 0.12	0.78 ± 0.23	0.33 ± 0.16	1.34 ± 0.11

^apRS313, empty plasmid control
^bStudent’s t-test indicated a significant difference between bmh1Δ and BY4741 at 50 mM KCl (P = 0.01)

ISSN: 1471-2164