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Table 5 RNA levels of PHO84 and SPL2 determined by qRT-PCR and complementation by wild type BMH1

From: Lack of 14-3-3 proteins in Saccharomyces cerevisiae results in cell-to-cell heterogeneity in the expression of Pho4-regulated genes SPL2 and PHO84

  RNA level (arbitrary units) (± SD; n = 3)
Strain PHO84 SPL2
50 mM 0 mM 50 mM 0 mM
BY4741 pRS313a 0.18 ± 0.03 1.10 ± 0.20 0.24 ± 0.12 1.50 ± 0.34
bmh1Δ pRS313 0.03 ± 0.01b 0.98 ± 0.31 0.05 ± 0.02 1.44 ± 0.21
BY4741 pRS313[BMH1] 1.01 ± 0.01 1.23 ± 0.40 1.05 ± 0.19 2.03 ± 0.22
bmh1Δ pRS313[BMH1] 0.18 ± 0.12 0.78 ± 0.23 0.33 ± 0.16 1.34 ± 0.11
  1. apRS313, empty plasmid control
  2. bStudent’s t-test indicated a significant difference between bmh1Δ and BY4741 at 50 mM KCl (P = 0.01)