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Fig. 8 | BMC Genomics

Fig. 8

From: Regulatory networks underlying mycorrhizal development delineated by genome-wide expression profiling and functional analysis of the transcription factor repertoire of the plant symbiotic fungus Laccaria bicolor

Fig. 8

Functional validation of L. bicolor transcriptional activators. Representative example of TAT results conducted on the six TFs similar to known function genes. Colonies were isolated from the TAT assay plates and analyzed by serial dilution assays (starting from an OD600 of 1.0) and 2 μl of each dilution were plated on selective plates. Resistance to 50 mM His3 enzyme inhibitor 3-amino-triazole (3-AT) and uracil prototrophy were used to assay the expression of the HIS3 and URA3 reporter genes. For the LacZ (β -Gal) gene reporter assay, 2 μl of yeast cell dilutions (OD600 = 0.1) were spotted on YPD plates overlaid by a nylon membrane, which were then incubated overnight at 30 °C, prior to β -galactosidase assay. Empty pDEST32 vector transformants were used as negative control; wt, m1 and m2 are internal assay controls

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BMC Genomics

ISSN: 1471-2164

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