Table 2 List of L. bicolor TFs, unconventional activators, and putative unconventional activators retrieved from, and functionally validated by, the TAT screen of FLMÂ +Â FB and ECM libraries
Sequence information | BlastP results | Conserved domains | ||||||
|---|---|---|---|---|---|---|---|---|
seq ID | Protein ID | FLM/FB | ECM | ACC number | Description | Organism | IPR number | Description |
Transcription factors (bearing a recognizable and specific DBD) | ||||||||
 Contig05 | 293242 | 59 | 10 | BAC55240.1 | C-Gcn4 | Candida maltosa | IPR011616 | bZIP |
 ECM-L2_G03 | 298274 | 0 | 1 | XP_001388805.1 | regulatory protein AbaA | Aspergillus niger | IPR000818 | TEA/ATTS |
 ECM-L1_F11 | 307744 | 0 | 1 | XP_002173151.1 | meiotically upregulated gene product | Schizosaccharomyces japonicus | IPR014778 | Myb |
 Contig62 | 386478 | 7 | 0 | XP_001830477.1 | PCC1 | Coprinopsis cinerea | IPR000910 | HMG1/HMG2 |
 Contig41 | 393192 | 3 | 0 | XP_001886200.1 | STE12-like | Laccaria bicolor | IPR013087 | C2H2 |
 ECM-L1_A03 | 457991 | 0 | 1 | XP_002910064.1 | NWD2 | Coprinopsis cinerea | IPR000253 | Forkhead |
 Contig40 | 458057 | 15 | 1 | XP_001383328.2 | ROX1-like HMG-box TF | Scheffersomyces stipitis | IPR000910 | HMG1/HMG2 |
 Contig65 | 481652 | 3 | 1 | XP_003501811.1 | RFX2 | Cricetulus griseus | IPR003150 | RFX |
 FLM-L2_D07 | 482609 | 1 | 0 | XP_001828950.2 | specific transcriptional repressor | Coprinopsis cinerea | IPR000910 | HMG1/HMG2 |
 FLM-L2_D05 | 486090 | 1 | 0 | XP_003034605.1 | expressed protein | Schizophyllum commune | IPR001138 | Zn2Cys6 |
 Contig46 | 628355 | 1 | 1 | XP_567555.1 | transcriptional regulatory protein | Cryptococcus neoformans | IPR007219 | Transcription factor, fungi |
 FLM-L2_A08 | 633206 | 1 | 0 | XP_001368548.1 | zinc finger protein 850-like | Musca domestica | IPR013087 | C2H2 |
 Contig43 | 640940 | 3 | 1 | AAC32736.1 | Prf1 | Ustilago maydis | IPR000910 | HMG1/HMG2 |
 Contig38 | 648888 | 6 | 3 | AAC32736.1 | Prf1 | Ustilago maydis | IPR000910 | HMG1/HMG2 |
 ECM-L1_H01 | 656449 | 0 | 1 | XP_001819986.2 | regulatory protein abaA | Aspergillus oryzae | IPR000818 | TEA/ATTS |
 Contig75 | 665554 | 2 | 0 | AAS64313.1 | Chap1 | Cochliobolus heterostrophus | IPR011616 | bZIP |
 Contig07 | 682475 | 2 | 0 | XP_001399919.1 | C6 transcription factor (Mut3) | Aspergillus niger | IPR001138 | Zn2Cys6 |
 Contig42 | 700637 | 16 | 5 | EGO20236.1 | hypothetical protein | Serpula lacrymans | IPR000433 | ZZ Zinc finger |
Proteins containing an aspecific nucleic acid binding domain | ||||||||
 Contig27 | 451329 | 0 | 2 | XP_002911693 | CAP-Gly domain-containing protein | Coprinopsis cinerea | IPR001878 | Zinc finger, CCHC-type |
 Contig39 | 585018 | 2 | 0 | XP_001836279.2 | hypothetical protein | Coprinopsis cinerea | IPR001606 | ARID/BRIGHT DNA-binding domain |
 Contig45 | 699941 | 2 | 0 | XP_001828564.2 | hypothetical protein | Coprinopsis cinerea | IPR019787 | Zinc finger, PHD-finger |
 FLM-L1_B12 | 640654 | 1 | 0 | XP_003507462.1 | hypothetical protein | Coprinopsis cinerea | IPR018957 | Zinc finger, C3HC4 RING-type |
 ECM-L1_E11 | 705628 | 0 | 1 | XP_002172787.1 | cps3 | Schizosaccharomyces japonicus | IPR000571 | Zinc finger, CCCH-type |
Unconventional activators with nuclear localization | ||||||||
 FLM-L3_C01 | 299583 | 1 | 0 | XP_001836340.2 | TKL/TKL-ccin protein kinase | Coprinopsis cinerea | IPR017442 | Serine/threonine-PK-like domain |
 FLM-L2_B06 | 300643 | 1 | 0 | CCA73543.1 | rec8- related meiotic recombination | Piriformospora indica | IPR006910 | Rad21/Rec8-like protein, N-terminal |
 ECM-L2_D08 | 468224 | 0 | 1 | XP_001828858.2 | Rad21 protein | Coprinopsis cinerea | IPR006909 | Rad21/Rec8-like protein, C-terminal |
 Contig70 | 459401 | 0 | 3 | XP_001833620.2 | ubiquitin-protein ligase | Coprinopsis cinerea | IPR000008 | C2 calcium-dependent membrane targeting |
 FLM-L2_F09 | 610588 | 1 | 0 | BAG24499.1 | rad57 | Coprinopsis cinerea | IPR013632 | DNA recombination and repair protein |
 Contig72 | 636246 | 0 | 2 | CCA72600.1 | EDE1-related, endocytosis | Piriformospora indica | IPR000449 | Ub-associated/transl elongation factor EF1B |
 ECM-L2_D05 | 685195 | 0 | 1 | NP_595780.1 | ribosome biogenesis protein Nop6 | Schizosaccharomyces pombe |  |  |
 ECM-L1_B01 | 698517 | 0 | 1 | XP_002472728.1 | 60S acidic ribosomal protein P1 | Postia placenta | IPR001813 | Ribosomal protein 60S |
 FLM-L2_D02 | 700143 | 1 | 0 | XP_001828708.2 | CMGC/RCK/MAK protein kinase | Coprinopsis cinerea | IPR017442 | Serine/threonine-PL-like domain |
Putative unconventional activators with intracellular localization | ||||||||
 Contig03 | 190404 | 0 | 3 | XP_001880663.1 | aspartic peptidase A1 | Laccaria bicolor | IPR001461 | Peptidase A1 |
 FLM-L1_F07 | 192523 | 1 | 0 | XP_001877048.1 | tubulin alpha | Laccaria bicolor | IPR003008 | Tubulin/FtsZ, GTPase domain |
 FLM-L2_D04 | 243371 | 1 | 0 | XP_002912138.1 | aconitate hydratase | Coprinopsis cinerea | IPR000573 | Aconitase A/isopropylmalate dehydratase |
 Contig36 | 294384 | 5 | 0 | XP_568826.1 | tubulin binding protein | Cryptococcus neoformans |  |  |
 ECM-L3_B06 | 324430 | 0 | 1 | XP_001877591.1 | copper transporter | Laccaria bicolor | IPR007274 | Ctr copper transporter |
 ECM-L3_A03 | 327303 | 0 | 1 | XP_001274183.1 | mitochondrial GTPase (YlqF) | Aspergillus clavatus | IPR023179 | GTP-binding protein |
 Contig01 | 444552 | 4 | 0 | XP_001835217.1 | peroxin19 | Coprinopsis cinerea | IPR006708 | Pex19 protein |
 ECM-L3_D03 | 521043 | 0 | 1 | CCA67049.1 | related to PDR16- lipid biosynthesis | Piriformospora indica | IPR001251 | Cellular retinaldehyde-binding |
 Contig09 | 583617 | 8 | 0 | CCA71746.1 | related to proteophosphoglycan ppg4 | Piriformospora indica |  |  |
 ECM-L2_F10 | 608638 | 0 | 1 | XP_001831367.1 | gamma-adaptin | Coprinopsis cinerea | IPR002553 | Clathrin/coatomer adaptor, adaptin-like |
 FLM-L2_H04 | 656953 | 1 | 0 | XP_001837070.1 | vacuole protein | Coprinopsis cinerea |  |  |
 FLM-L2_E08 | 659644 | 1 | 0 | XP_001839900.1 | elongation factor 3 | Coprinopsis cinerea | IPR015688 | Elongation Factor 3 |
 FLM-L2_E09 | 671307 | 1 | 0 | XP_001830051.1 | peroxisomal targeting signal 1 receptor | Coprinopsis cinerea | IPR001440 | Tetratricopeptide TPR-1 |
 FLM-L2_D03 | 695354 | 1 | 0 | XP_001840019.1 | mitochondrial carrier protein | Coprinopsis cinerea | IPR018108 | Mitochondrial substrate/solute carrier |
 ECM-L2_E11 | 703237 | 0 | 1 | XP_002911229.1 | rho GDP-dissociation inhibitor | Coprinopsis cinerea | IPR000406 | RHO protein GDP dissociation inhibitor |
 FLM-L2_A07 | 707485 | 1 | 0 | XP_002910841.1 | guanine nucleotide exchange factor | Coprinopsis cinerea | IPR003123 | Vacuolar sorting protein 9 |
 ECM-L1_B05 | 311818 | 0 | 1 | XP_003037815.1 | hypothetical protein | Schizophyllum commune |  |  |
 FLM-L2_F11 | 321043 | 1 | 0 | XP_001829416.1 | hypothetical protein CC1G_00595 | Coprinopsis cinerea |  |  |
 ECM-L2_D01 | 325350 | 0 | 1 | P_001837732.1 | hypothetical protein CC1G_06938 | Coprinopsis cinerea |  |  |
 FLM-L2_H02 | 390988 | 1 | 0 | XP_001841219.1 | hypothetical protein CC1G_11382 | Coprinopsis cinerea |  |  |
 FLM-L1_C04 | 439929 | 1 | 0 | EGO18585.1 | hypothetical protein | Serpula lacrymans |  |  |
 Contig32 | 459061 | 0 | 3 | XP_003026161.1 | hypothetical protein | Schizophyllum commune |  |  |
 Contig10 | 509577 | 1 | 1 | XP_001882083.1 | predicted protein | Laccaria bicolor |  |  |
 FLM-L3_F02 | 546684 | 1 | 0 | EGO29111.1 | hypothetical protein | Serpula lacrymans |  |  |
 Contig54 | 549772 | 4 | 1 | XP_001833470.2 | hypothetical protein CC1G_05170 | Coprinopsis cinerea |  |  |
 Contig76 | 576504 | 5 | 0 | XP_001828840.2 | hypothetical protein | Coprinopsis cinerea |  |  |
 Contig37 | 604174 | 2 | 0 | XP_001834463.1 | hypothetical protein CC1G_02199 | Coprinopsis cinerea |  |  |
 Contig24 | 613652 | 2 | 0 | XP_001830379.1 | hypothetical protein | Coprinopsis cinerea |  |  |
 ECM-L3_B04 | 622202 | 0 | 1 | XP_001828856.1 | hypothetical protein CC1G_03650 | Coprinopsis cinerea |  |  |
 ECM-L2_E05 | 626440 | 0 | 1 | NP_587684.1 | hypothetical protein | Coprinopsis cinerea | IPR019350 | RNA polymerase I-specific transcription initiation factor RRN6-like |
 FLM-L1_G10 | 634434 | 1 | 0 | XP_001831375.1 | hypothetical protein CC1G_00922 | Coprinopsis cinerea |  |  |
 FLM-L2_F03 | 656382 | 1 | 0 | XP_001875331.1 | predicted protein | Laccaria bicolor |  |  |
 Contig78 | 680010 | 5 | 0 | XP_002476516.1 | predicted protein | Postia placenta |  |  |
 FLM-L2_E07 | 686252 | 1 | 0 | XP_001836432.2 | hypothetical protein CC1G_07079 | Coprinopsis cinerea |  |  |
 ECM-L3_C02 | 688275 | 0 | 1 | XP_001835642.2 | hypothetical protein CC1G_03424 | Coprinopsis cinerea | IPR016021 | MIF4-like, type 1/2/3 |
 Contig58 | 693322 | 0 | 4 | XP_001830639.2 | hypothetical protein CC1G_06905 | Coprinopsis cinerea | IPR003864 | Domain of unknown function DUF221 |
 ECM-L1_B02 | 693899 | 0 | 1 | EGO22612.1 | hypothetical protein | Serpula lacrymans |  |  |
 FLM-L1_E04 | 708222 | 1 | 0 | XP_003036324.1 | expressed protein | Schizophyllum commune |  |  |
 Contig68 | 708574 | 0 | 2 | XP_001833764.1 | hypothetical protein | Coprinopsis cinerea |  |  |
Secreted protein | ||||||||
 ECM-L1_H02 | 394934 | 0 | 1 | ZP_01463678.1 | sphingolipid ceramide N-deacylase | Stigmatella aurantiaca |  |  |
 Contig44 | 643792 | 3 | 0 | XP_001836617.1 | hypothetical protein CC1G_06204 | Coprinopsis cinerea |  |  |
 FLM-L3_H05 | 658920 | 1 | 0 | XP_001830283.1 | hypothetical protein CC1G_01919 | Coprinopsis cinerea |  |  |
 FLM-L2_G08 | 660445 | 1 | 0 | XP_001835021.1 | hypothetical protein CC1G_09912 | Coprinopsis cinerea | IPR018499 | Tetraspanin |
 FLM-L1_G04 | 688063 | 1 | 0 | XP_001835466.2 | hypothetical protein CC1G_05428 | Coprinopsis cinerea |  |  |
Secreted proteins with nuclear localization signal (STAPs) | ||||||||
 FLM-L1_A04 | 304792 | 1 | 0 | XP_001840014.1 | hypothetical protein CC1G_10398 | Coprinopsis cinerea |  |  |
 FLM-L1_F01 | 391051 | 1 | 0 | XP_001840014.1 | hypothetical protein | Coprinopsis cinerea |  |  |
 FLM-L1_D07 | 455116 | 1 | 0 | XP_001884865.1 | predicted protein | Laccaria bicolor |  |  |
 Contig64 | 659547 | 77 | 1 | AAD01986.1 | ras related protein | Laccaria bicolor |  |  |