Table 2 Primers used in quantitative real-time PCR analysis
From: ITRAQ-based quantitative proteomic analysis of Cynops orientalis limb regeneration
Target gene | Primer | Sequence (5’to3’) |
|---|---|---|
Epidermal differentiation-specific protein | EDSP_F | GTGGAGCTGAAAATCGTCC |
EDSP_R | CATCAATGTAAGTTTCTGTGCC | |
Carboxypeptidase Z | CPZ_F | GATTAAGCCTGGTCGGGTAG |
CPZ_R | TGGGTCGAACTGATCTAACG | |
Cadherin-13 | CDH13_F | AAGCCTTTGGACTACGAGA |
CDH13_R | ATGGACCTTCATTGACATCAC | |
Macrophage-capping protein | CAPG_F | TGACTGAACACGGGAAGG |
CAPG_R | CAATGCTGGAGATGACGC | |
Coronin-1A | CORO1A_F | GCTTTGATGAACCATTGACG |
CORO1A_R | TGTGACTCCTTGCTGCTGTAC | |
Basement membrane-specific heparan sulfate proteoglycan core protein | HSPG2_F | CAATACTGTGAGCGATGTGC |
HSPG2_R | ACTTGAACGATGAAAGGAGC | |
Annexin A1 | ANXA1_F | TTACGAAACTATTCTGGTGGC |
ANXA1_R | TTTTGGGATTACAGGCACA | |
eukaryotic translation initiation factor 5A isoform II | EIF5A2_F | AAGATACGAGGCAGGAGAAG |
EIF5A2_R | AAGTGGGCGATTATTTGGT | |
Cathepsin B | CTSB_F | CTGTCTTGCTGTGGTCTGG |
CTSB_R | AGGTTGTCACGCATTTGG | |
Beta-actin | ACTB_F | CTCAACCCCAAAGCCAATC |
ACTB_R | GAAGCATACAGGGACAGCA | |
Hemopexin | HPX_F | CGCTCGGATCAGCTCTACAC |
| Â | HPX_R | TTCAGGTCCACCAAAGAAAGG |
Integrin beta-4 | ITGB4_F | TTCGCTTGACCAATGATGTAGA |
| Â | ITGB4_R | GGCTTCGTAGTGAAATGCTGAC |
Collagen alpha-1(II) chain | COL2A1_F | CCTGGAACACCTGGCACTG |
| Â | COL2A1_R | TCACCTACGTCACCCCTGTCT |