Fig. 4

Global overview of the dynamic changes in primary metabolite pool sizes during deacclimation of Arabidopsis thaliana. Plants were non-acclimated (NA) or cold acclimated at 4 °C for 3 d (ACC). Cold acclimated plants were then deacclimated (DEA) at 20 °C for 2 h, 4 h, 6 h, 12 h and 24 h. The metabolic profiles were statistically analyzed using a one-way ANOVA at FDR P < 0.05 by comparing all conditions against each other. Metabolites that showed significant changes between treatments were clustered using Euclidian distance and average linkage. Lists with the names of all metabolites in the different clusters indicated by the letters a to f can be found in Additional file 7. The numbers on the right side of the heat map refer to the numbering of the metabolites in Additional file 3