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Table 1 List of large-scale chromosomal deletion mutants

From: Developing genome-reduced Pseudomonas chlororaphis strains for the production of secondary metabolites

Mutant

Multiple-deletion (bp)

Cumulative deletion

Genomic loci

Description

(bp)

(%)

MDS1

14,022

14,022

0.21

MOK_02716-MOK_02727

achromobactin operon

MDS2

21,614

35,636

0.53

MOK_04727-MOK_04735

P.fluorescens insecticidal toxin operon

MDS3

48,164

83,800

1.26

MOK_01688-MOK_01707

part of pvd operon

MDS4

2548

86,348

1.30

MOK_04926,MOK_04927

hydrogen cyanide operon

MDS5

6726

93,074

1.40

MOK_04030-MOK_04036

pyrrolnitrin operon

MDS6

19,167

112,241

1.68

MOK_00110-MOK_00130

includes ccdA, azlC, tdcB, gss. mgtA

MDS7

41,371

153,612

2.31

MOK_00373-MOK_00408

includes ltaA, gbcAB, proXWV, betTIBA

MDS8

18,221

171,833

2.58

MOK_00955-MOK_00974

includes aurF, fabGZF, acpP, psyIR, ada

MDS9

24,416

196,249

2.95

MOK_00497-MOK_00507

includes pvdL, pvdS, atuR, atuA

MDS10

38,000

234,249

3.52

MOK_00047-MOK_00091

includes ligT, mmsB, araD, hyi, folA

MDS11

26,771

261,020

3.92

MOK_01641-MOK_01663

includes katG, vgrG, gstA, amiE, surE

MDS12

37,633

298,653

4.48

MOK_01720-MOK_01754

includes str, pea operon, aphA_2, mrdA_1

MDS13

37,411

336,064

5.04

MOK_01897-MOK_01932

includes matP, wbp operon, lpd3, cdsA_2

MDS14

29,156

365,220

5.48

MOK_02207-MOK_02237

includes hisPMQJ, dam

MDS15

24,108

389,328

5.84

MOK_02626-MOK_02642

includes dmpA, apr operon, pspAB, pueBA

MDS16

28,273

417,601

6.27

MOK_02657-MOK_02685

includes ssuBCA, aidB, uspA, puuC, dehII

MDS17

87,173

504,774

7.58

MOK_02743-MOK_02813

includes fabG, idnO, aofH, aidB, phoD, aofH

MDS18

26,866

531,640

7.98

MOK_02690-MOK_02714

includes gabT, narL, glnA_3, plcN, FecR

MDS19

28,745

560,385

8.41

MOK_03695-MOK_03722

mostly related to hypothetical protein

MDS20

56,152

616,537

9.25

MOK_05764-MOK_05802

mostly related to hypothetical protein

MDS21

40,441

656,978

9.86

MOK_01057-MOK_01097

czcABCRD and genes with unknown function

MDS22

28,772

685,750

10.29

MOK_00230-MOK_00246

mostly encoding hypothetical protein