Developing genome-reduced Pseudomonas chlororaphis strains for the production of secondary metabolites

BMC Genomics

Table 2 Fermentation characteristics of the parental strain GP72(rpeA-) and the genome-reduced mutants MDS10 and MDS22

Strain	Specific growth rate (h⁻¹)	Specific PCA production rate(mmol/g/h)	Specific 2-OH-PCA production rate(mmol/g/h)	Specific 2-OH-PHZ-24 production rate(mmol/g/h)	Specific total phenazine production rate (g/g/h)^a
GP72(rpeA-)	0.1226 ± 0.0034	0.0061 ± .00031	0.0064 ± 0.00024	0.0008 ± 0.00002	0.0031 ± 0.00011
MDS10	0.0601 ± .00360	0.0202 ± .00067	0.0015 ± 0.00010	0.0035 ± 0.00014	0.0056 ± 0.00019
MDS22	0.0314 ± .00280	0.0076 ± .00075	0.0025 ± 0.00015	0.0092 ± 0.00040	0.0039 ± 0.00025

Data represent the mean ± SD from three independent cultures
^aBecause the molecular weights of PCA, 2-OH-PCA and 2-OH-PHZ are different, the specific total phenazine production rate was calculated in grams

ISSN: 1471-2164