Table 1 Summary of RNA-seq quality, read counts, mapping rates and transcript assemblies for 12 libraries
Librarya | Clean read number | Number of clean bases (Gb) | Percentage of Q20 bases (%) | Read mapping rate (%) | Unique hits to reference genome (%) | Number of transcripts | Percentage of annotated transcripts (%) | Mean length (bp) of transcripts | N50 (bp) |
|---|---|---|---|---|---|---|---|---|---|
BSD1_1 | 33,901,938 | 4.28 | 95.6 | 59.6 | 95.7 | 22,696 | 71.5 | 1746 | 2813 |
BSD1_2 | 30,474,832 | 3.71 | 96.9 | 56.2 | 95.9 | 25,558 | 76.1 | 1784 | 2703 |
BSD1_3 | 31,658,176 | 3.80 | 95.8 | 55.7 | 96.0 | 28,311 | 72.7 | 1723 | 2589 |
WSD1_1 | 34,289,474 | 4.33 | 95.9 | 59.9 | 96.5 | 29,876 | 74.3 | 1917 | 3017 |
WSD1_2 | 30,571,674 | 3.88 | 96.7 | 54.5 | 94.9 | 31,958 | 71.6 | 1972 | 2923 |
WSD1_3 | 36,218,956 | 4.58 | 96.1 | 60.9 | 96.5 | 29,373 | 74.1 | 1925 | 2996 |
BSD2_1 | 44,488,720 | 6.67 | 97.0 | 54.6 | 97.2 | 29,867 | 72.2 | 1823 | 2771 |
BSD2_2 | 44,208,500 | 6.63 | 97.1 | 50.8 | 96.3 | 28,969 | 73.3 | 1936 | 3025 |
BSD2_3 | 66,474,016 | 9.97 | 96.9 | 55.2 | 97.0 | 39,296 | 66.2 | 1901 | 3005 |
WSD2_1 | 48,196,678 | 7.23 | 96.2 | 52.0 | 99.2 | 38,065 | 67.6 | 1779 | 2832 |
WSD2_2 | 45,360,764 | 6.80 | 95.6 | 59.1 | 99.2 | 37,560 | 67.7 | 1755 | 2752 |
WSD2_3 | 45,907,664 | 6.89 | 96.1 | 52.4 | 99.3 | 36,978 | 68.7 | 1811 | 2764 |
Mean ± SD | 40,979,283 ± 10,406,949 | 5.73 ± 1.93 | 96.3 ± 0.6 | 55.9 ± 3.3 | 96.9 ± 1.5 | 31,542 ± 5308 | 71.3 ± 3.1 | 1839 ± 86 | 2849 ± 142 |