Fig. 5

Modeling of random-mer barcode errors. Barcode error probabilities in the random-mer barcode sequence were modeled as being comprised of either (a) errors arising during PCR, or (b) errors arising during the sequencing assay. Error probabilities are calculated per initial library molecule. A simple model based on Poisson statistics was used; details are described in Methods. c Number of distinct random-mer sequences found in each EXB read group. The number of distinct random-mer sequences is measured as a function of the EXB read group size. Dots indicate the mean and the shaded area indicates the 5 and 95% quantiles. One million reads from each library are pooled and grouped by paired-end EXB sequence. d Edit distance between distinct random-mer sequences found in each EXB read group. The edit distance between distinct random-mer sequences is measured as the maximum edit distance between all random-mer sequences in a single EXB read group. Dots indicate the mean and the shaded area indicates the 5 and 95% quantiles