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Fig. 2 | BMC Genomics

Fig. 2

From: An analysis of 67 RNA-seq datasets from various tissues at different stages of a model insect, Manduca sexta

Fig. 2

Overview of the 67 cDNA libraries. a Total read numbers in the libraries. As defined in Fig. 1, bar colors represent the tissue sources of libraries 1–67. Black and cyan IDs indicate the libraries were determined by paired- and single-end sequencing, respectively. b Up-boundaries represent percentages of the total reads after trimming (green) and mapping by STAR (yellow) and TopHat (blue), with the total reads (grey) in each library set at 100%. The library names and their color codes are the same as in Fig. 1. c and d. Box-plots of survived read numbers and percentages after trimming in categories P (paired-end, 33 of the first 52 libraries), S (single-end, 19 of libraries 1–52), H (head, single-end, 53–60), and A (antenna, single-end, 61–67). e Percentages of trimming-survived reads mapped to the genome using STAR and TopHat in the four categories. f Percentages of TopHat-mapped reads corresponding to mitochondrial (blue), protein-coding (white), noncoding (green), and rRNA (red) genes. g and h Box-plots of percentages of trimming-survived reads mapped to mitochondrial and rRNA genes in categories P, S, H and A. The first 52 libraries were sequenced as a part of the genome project [28], the next 8 were for detecting sex-biased genes expression in brain [14], and the last 7 were used to study chemosensory receptor gene expression [27]

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