Fig. 2

Quantitative real-time PCR (qRT-PCR) validation of 12 differentially expressed genes. Accumulations of 12 genes were analyzed by qRT-PCR using EF1α as internal control under dehydration stress for 0, 1, and 5 h. Data was shown as means ± S.D. (n = 4). White and gray bars represented for qRT-PCR results and RNA-Seq data, respectively. Gene-specific primers used for real-time PCR were listed in Additional file 1