Table 4 List of key genes differentially regulated between the 14dpa and 30dpa stages
S. No | Metabolism involved | Pathway involved | Key regulatory gene differentially expressed | Stages expressed |
|---|---|---|---|---|
1 | Nucleotide metabolism | Aminoacyl-tRNA biosynthesis | Ligase | D14 and D30 |
2 | Nucleotide metabolism | Aminoacyl-tRNA biosynthesis | Synthase (Glutamine-hydrolysing) | D30 |
3 | Amino acid metabolism | Phenylalanine metabolism | Lactoperoxidase | U14, D14, D30 and U30 |
4 | Amino acid metabolism | Histidine; Lysine; Arginine and proline; cysteine and methionine; alanine, aspartate and glutamate; phenylalanine, tyrosine and tryptophan metabolism | Transaminase | D14 |
5 | (Photorespiration; T-subunit of GDS) | Glycine, Serine and Threonine metabolism | S-aminomethyl dihydro lipoylprotein:(6S)-tetrahydrofolate aminomethyltransferase (ammonia-forming) | D14 and D30 |
6 | (Photorespiration; P-subunit of GDS) | Glycine, Serine and Threonine metabolism | dehydrogenase (aminomethyl-transferring) | D14 |
7 | Carbohydrate metabolism | Fructose and mannose; and galactose metabolism | Phosphohexokinase | D14 and D30 |
8 | Carbohydrate metabolism | Amino sugar and nucleotide sugar; fructose and mannose; galactose; and starch and sucrose metabolism | Hexokinase type IV glucokinase | D14 and D30 |
9 | (Photorespiration) | Glyoxylate and dicarboxylate metabolism (under carbohydrate metabolism) | Transaminase | D14 |
10 | Respiratory pathways | Pentose phosphate pathway; Glycolysis / gluconeogenesis metabolism | Phosphohexokinase | D14 and D30 |
11 | Respiratory pathways | Glycolysis / gluconeogenesis metabolism | Hexokinase type IV glucokinase | D14 and D30 |
12 | Photosynthesis | Porphyrin and chlorophyll metabolism | protoporphyrin IX methyltransferase | D14 |
13 | Photosynthesis | Porphyrin and chlorophyll metabolism | Porphobilinogen synthase | D14 |
14 | Photosynthesis | Porphyrin and chlorophyll metabolism | Protoporphyrinogen oxidase | D14 |
15 | Photosynthesis | Carbon fixation in photosynthetic organisms | Carboxylase (RuBisCO) | U14, D14, and D30 |
16 | Photosynthesis | Carbon fixation in photosynthetic organisms | Carboxylase (phosphoenolpyruvate) | D14 and D30 |
17 | Photosynthesis | Carbon fixation in photosynthetic organisms | Transaminase (Aspartate; EC2..6.1.1) | D14 |
18 | Photosynthesis | Carbon fixation in photosynthetic organisms | Dehydrogenase (malate) | D14 and D30 |
19 | Photosynthesis | Carbon fixation in photosynthetic organisms | Dehydrogenase (decarboxylating) | D14 and D30 |
20 | Photosynthesis | Carbon fixation in photosynthetic organisms | Transaminase (alanine; EC2.6.1.2) | D14 and D30 |
21 | Photosynthesis | Carbon fixation in photosynthetic organisms | Phosphate dikinase | D14 |
22 | Lipid metabolism | Fatty acid biosynthesis; fatty acid degradation | Ligase (EC6.2.1.3) | D14 |
23 | Lipid metabolism | Fatty acid biosynthesis | Hydrolase (EC3.1.2.14) | D14 |
24 | Lipid metabolism | Fatty acid elongation | Hydrolase (EC3.1.2.22) | D14 |
25 | Lipid metabolism | Glycerophospholipid metabolism; ether lipid metabolism; arachidonic acid metabolism; linoleic acid metabolism; alpha-linolenic acid metabolism | Phospholipase A2 | D14 and D30 |
26 | Hormone biosynthesis | Steroid biosynthesis | Monooxygenase | D14 |
27 | Vitamin metabolism | One carbon pool by folate | Reductase | D30 |
28 | Specialized metabolism | Phenylpropanoid metabolism | Ammonia-lyase | D14 and D30 |
29 | Specialized metabolism | Terpenoid backbone biosynthesis | Geranyl-diphosphate synthase | D14 and D30 |