Fig. 6

Minimal input RNA mass for optimal T7SL IVT. a Graph showing the correlation between the input total RNA mass (X axis) and the aRNA yield (Y axis). b aRNA length distribution obtained with different input RNA mass. Note that below 6.25 ng input, the aRNA lose the typical smooth length distribution. For all samples, the same RNA mass were applied on a BioAnalyzer chip. c RNA-Seq scatterplot with different RNA inputs (from 100 to 3 ng) for T7SL IVT. Pearson correlation is depicted, based on log normalized read counts. Note that even when 3 ng of mass input is used for RNA amplification, the transcriptome quantification is very similar across all expression levels. “Sort” sample correspond to 10⁵ epimastigotes sorted directly to RNA extraction buffer, which roughly corresponds to 68 ng of total RNA (Additional file 4, Table S2)