From: Expression-based clustering of CAZyme-encoding genes of Aspergillus niger
Substrate | Conc. | Company | Purity | Composition |
---|---|---|---|---|
D-glucose | 25 mM | Sigma-Aldrich | ≥99.5% | – |
D-fructose | 25 mM | Sigma-Aldrich | ≥99% | – |
D-galactose | 25 mM | Sigma-Aldrich | ≥99% | – |
L-arabinose | 25 mM | Sigma-Aldrich | ≥99% | – |
D-xylose | 25 mM | Sigma-Aldrich | ≥99% | – |
D-mannose | 25 mM | Sigma-Aldrich | ≥99% | – |
D-galacturonic acid | 25 mM | Sigma-Aldrich | ≥99% | – |
L-rhamnose | 25 mM | Sigma-Aldrich | ≥99% | – |
Maltose | 25 mM | Sigma-Aldrich | ≥99% | – |
Sucrose | 25 mM | Sigma-Aldrich | ≥99% | – |
Ferulic acid | 25 mM | Sigma-Aldrich | ≥99% | – |
Inulin from chicory | 1% | Sigma-Aldrich | ns | ns |
Cellulose | 1% | Sigma-Aldrich | ns | ns |
Guar gum | 1% | Sigma-Aldrich | ns | ns |
Xyloglucan | 1% | Sigma-Aldrich | ns | ns |
Xylan (from beechwood) | 1% | Sigma-Aldrich | >90% | >90% D-xylose residues |
Polygalacturonic acid (from apples) | 1% | Sigma-Aldrich | ≥85% | ns |
Apple pectin | 1% | Sigma-Aldrich | ns | ns |
Galactan (from potato) | 1% | Megazyme | ns | Gal: Ara: Rha: GalAÂ =Â 88: 2: 3: 7 |
Debranched 1,5-α-L-arabinan (from sugar beet) | 1% | Megazyme | ~95% | Ara: Gal: Rha: GalA = 88: 4: 2: 6 |
Rhamnogalacturonan I (from potato) | 1% | Megazyme | >97% | GalA: Rha: Ara: Xyl: Gal: Os = 62: 20: 3.3: 1: 12: 1.7 |
Mannan (ivory nut) | 1% | Megazyme | >98% | 99% Mannan, Ara + Xyl traces |
Citrus pulp | 1% | ns | ns | Glc: GalA: Ara: Gal: Xyl: Man: Rha = 39: 35: 11: 7: 4: 4: 1 |
Sugar beet pulp | 1% | ns | ns | Glc: Ara: GalA: Gal: Xyl: Man: Rha = 33: 28: 26: 7: 2: 2: 1 |
Soy bean hulls | 1% | ns | ns | Glc: GalA: Xyl: Ara: Man: Gal: Rha = 49: 16: 15: 8: 7: 4: 1 |