De novo assembly of genomes from long sequence reads reveals uncharted territories of Propionibacterium freudenreichii

Table 2 Sequencing summary

Sample Name	Sequencing Chemistry	SMRTcells	Movie Time (min) ^a	Total Number of Subreads	Total Number of Bases	Mean Subread Length (bp)	N50 Subread Length (bp) ^b
JS4	P4/C2	2	120	142,306	465,129,578	3268	4131
JS10	P4/C2	2	120	205,113	606,802,054	2958	3733
JS15	P4/C2	2	120	179,642	540,206,310	3007	3653
JS18	P4/C2	2	120	194,246	550,199,736	2832	3679
JS20	P4/C2	2	120	230,371	632,635,668	2746	3456
JS22	P4/C2	2	120	181,361	563,541,807	4090	3107
JS23	P4/C2	2	120	192,373	617,310,336	3208	4222
JS2	P5/C3	2	240	100,864	719,516,424	7133	8940
JS7	P5/C3	2	240	107,194	1,002,978,417	9356	13,043
JS8	P5/C3	2	240	139,418	1,149,552,255	8245	11,148
JS9	P5/C3	2	240	160,808	1,316,084,462	8184	11,023
JS11	P5/C3	2	240	184,744	1,422,323,823	7698	10,498
JS12	P5/C3	2	240	207,391	1,490,421,212	7186	9765
JS13	P5/C3	2	240	103,466	793,823,369	7672	10,156
JS14	P5/C3	2	240	125,130	966,521,587	7724	10,326
JS17	P5/C3	2	240	151,002	1,149,295,071	7611	10,232
JS21	P5/C3	2	240	173,843	1,298,367,718	7468	9906
JS25	P5/C3	2	240	192,104	1,293,994,542	6735	8805

^a run time during which nucleotides added by the polymerase are recorded in real-time
^b minimum length of subreads in which half of the sequencing data is found

ISSN: 1471-2164