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Table 1 Biological data corresponding to the 11 small RNA libraries used in the present work

From: Comparative analysis of miRNA expression during the development of insects of different metamorphosis modes and germ-band types

Library Developmental period Age (AO: after oviposition) Percentage of embryo development (ED) Embryo development and Tanaka (1976) stage Hormonal context
NFE Non-fertilized egg Day 8 of the first gonadotrophic cycle (in preoviposition) -- Not determined within the egg. High levels of 20E and JH in the surrounding haemolymph.
ED0 Embryo 8 h AO, when the ootheca is still vertical 2%. Only yolk granules observed. No detectable levels of 20E and JH
ED1 Embryo 24 h AO 6% Energids at low density spread among the yolk granules. Tanaka stage 1 No detectable levels of 20E and JH
ED2 Embryo 48 h AO 12% Abundant energids, germ band anlage well delimited, slightly expanded at both sides. Tanaka stage 2 Burst of 20E inferred from the expression of HR3 (a 20E–dependent gene). No detectable JH
ED6 Embryo 144 h AO 33% Pleuropodia well apparent, legs segmented, caudal space arises. Tanaka stage 8 Peak of 20E. Very low levels of JH
ED13 Embryo 312 h AO 72% Eyes well colored, antennae and legs reaching the 5th abdominal segment. Tanaka stage 15 Peak of 20E. High levels of JH
N1 1st nymphal instar 1–2 days old High levels of 20E and JH
N3 3rd nymphal instar 2–4 days old High levels of 20E and JH
N5 5th nymphal instar 3–5 days old High levels of 20E and JH
N6 6th nymphal instar 5–7 days old High levels of 20E, no JH
Adult Adult (female) 5 days old Low (ovarian) levels of 20E and high JH levels