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Table 2 Evaluation of the GH classes in T. harzianum

From: Carbohydrate-active enzymes in Trichoderma harzianum: a bioinformatic analysis bioprospecting for key enzymes for the biofuels industry

GH family

Number

Principal enzyme activitya

EC numbera

RNA-Seq (TPM)b

    

CEL

DSB

LAC

GH1

4

β-glucosidase

3.2.1.21

26,464.6

24,945.6

25,780.2

GH2

13

β-mannosidase

3.2.1.25

10,109.9

10,652.8

10,615.0

GH3

17

β-glucosidase

3.2.1.21

23,132.5

24,232.1

24,991.6

GH4

1

α-glucosidase

3.2.1.22

1405.5

1866.2

1500.3

GH5

12

Endo- β-1,4-glucanase

3.2.1.4

15,970.5

17,669.5

16,084.8

GH6

1

cellobiohydrolase

3.2.1.91

35,003.8

46,112.3

48,148.5

GH7

2

Endo- β-1,4-glucanase

3.2.1.4

56,503.3

62,881.9

73,957.0

GH10

2

Endo-1,4- β-xylanase

3.2.1.8

46,748.0

61,201.9

53,469.7

GH11

4

Endo-1,4- β-xylanase

3.2.1.8

53,279.5

69,046.4

64,929.2

GH12

3

Endo- β-1,4-glucanase

3.2.1.4

10,362.0

12,937.4

10,779.4

GH13

8

α-amylase

3.2.1.1

5028.8

4708.1

4757.5

GH15

2

Glucoamylase

3.2.1.3

3776.3

3476.4

3103.1

GH16

16

Xyloglucanase

3.2.1.151

59,203.1

56,083.1

62,280.8

GH17

4

Endo-1,3- β-glucosidase

3.2.1.39

11,593.9

11,757.2

13,578.5

GH18

23

Chitinase

3.2.1.14

63,673.6

84,188.2

93,866.4

GH20

3

β-hexosaminidase

3.2.1.52

35,521.8

33,517.7

23,889.0

GH23

1

Lysozyme

3.2.1.17

1227.2

849.4

1093.1

GH24

1

Lysozyme

3.2.1.17

2492.6

1450.6

1586.9

GH25

1

Lysozyme

3.2.1.17

1885.9

1657.9

1559.4

GH26

2

β-mannanase

3.2.1.78

314.0

315.2

308.3

GH27

9

α-galactosidase

3.2.1.22

1524.0

1354.3

1373.8

GH28

3

polygalacturonase

3.2.1.15

38,662.7

26,783.2

27,177.1

GH30

6

Endo- β-1,4-xylanase

3.2.1.8

4233.3

3652.1

4332.6

GH31

5

α-glucosidase

3.2.1.20

6161.9

5753.5

7524.8

GH32

2

Invertase

3.2.1.26

0.0

0.0

0.0

GH33

1

neuraminidase

3.2.1.18

0.0

0.0

0.0

GH35

1

β-galactosidase

3.2.1.23

1814.5

1099.7

1961.4

GH36

2

α-galactosidase

3.2.1.22

308.4

255.6

240.4

GH37

2

α-trehalase

3.2.1.28

2379.5

1017.6

1640.6

GH38

1

α-mannosidase

3.2.1.24

964.8

1116.1

960.2

GH39

3

β-xylosidase

3.2.1.37

648.2

444.7

446.0

GH43

5

β-xylosidase

3.2.1.37

3643.7

3004.4

4153.1

GH45

3

Endoglucanase

3.2.1.4

4845.5

4891.1

4353.0

GH47

6

α-mannosidase

3.2.1.113

8278.3

6385.7

7684.9

GH49

1

Dextranase

3.2.1.11

0.0

0.0

0.0

GH54

2

α-L-arabinofuranosidase

3.2.1.55

1253.1

1234.0

2333.9

GH55

9

Endo- β-1,3-glucanase

3.2.1.39

3113.4

2662.3

3444.8

GH62

2

α-L-arabinofuranosidase

3.2.1.55

9919.7

8963.6

14,790.8

GH63

1

α-glucosidase

3.2.1.106

812.5

509.6

521.8

GH64

3

β-1,3-glucanase

3.2.1.39

9176.4

8197.0

8943.6

GH65

1

α-trehalase

3.2.1.28

1585.3

1273.2

1085.3

GH67

2

α-glucuronidase

3.2.1.139

1300.6

732.9

1057.3

GH71

6

α-1,3-glucanase

3.2.1.59

13,633.2

15,112.4

16,804.9

GH72

5

β-1,3-glucanosyltransglycosylase

2.4.1-

26,604.3

25,458.0

26,995.4

GH75

5

Chitosanase

3.2.1.132

3030.0

4812.3

4033.0

GH76

9

α-1,6-mannanase

3.2.1.101

14,462.0

11,978.6

10,448.3

GH78

3

α-L-rhamnosidase

3.2.1.40

111.9

44.6

55.3

GH79

3

β-glucuronidase

3.2.1.31

299.1

232.8

219.4

GH81

2

Endo- β-1,3-glucanase

3.2.1.39

244.0

164.1

137.3

GH88

2

d-4,5-unsaturated β-glucuronyl hydrolase

3.2.1

263.6

100.3

80.0

GH89

2

α-N-acetylglucosaminidase

3.2.1.50

1059.2

760.0

533.5

GH92

8

Mannosyl-oligosaccharide α-1,2-mannosidase

3.2.1.113

7002.9

5376.4

5435.1

GH93

4

Exo- α-L-1,5-arabinanase

3.2.1

7736.1

4164.5

4140.9

GH95

5

α-L-fucosidase

3.2.1.51

680.7

554.0

602.6

GH105

1

Unsaturated rhamnogalacturonyl hydrolase

3.2.1.172

0.0

0.0

0.0

GH114

1

Endo- α-1,4-polygalactosaminidase

3.2.1.109

2290.2

1929.7

1214.4

GH115

1

Xylan α-1,2-glucuronidase

3.2.1.131

3420.7

3848.9

3455.7

GH125

2

Exo- α-1,6-mannosidase

3.2.1

3221.8

2177.3

2401.5

GH127

1

β-L-arabinofuranosidase

3.2.1.185

628.6

399.2

378.0

GH128

4

β-1,3-glucanase

3.2.1.39

5748.1

3170.0

3356.8

GH132

2

Activity on β-1,3-glucan

–

4211.0

5376.7

5835.3

GH133

1

Amylo- α-1,6-glucosidase

3.2.1.33

1480.5

1058.1

1132.8

GH135

2

α-1,4-galactosaminogalactan hydrolase

3.2.1

153.7

134.0

121.9

  1. TPM, transcripts per million
  2. aCAZy database ( www.cazy.org )
  3. bThe expression values for the different GH families were calculated by summing the level of TPM expression in each gene composing the family