Fig. 2

Defense genes are highly enriched in SR45-downregulated genes. a. Venn Diagrams showing SR45-upregulated and -downregulated genes identified by three different pipelines. b. A sample of GO enrichment analysis for SR45-downregulated genes that are commonly identified by all three pipelines. Fold of enrichment = ratio of Actual/Expected Gene numbers. c-d. qRT-PCR confirmation of selected SR45-differentially regulated genes. Relative expression of each gene is defined as 1 in Col-0. GAPDH was used as control. Poly(A)-mRNAs prepared from inflorescence were used to perform qRT-PCR in each sample as described in Methods. n = 3. Error bars present standard deviations. Student t-test was used to compare the relative expression between Col-0 and sr45–1. * p < 0.05, ** p < 0.01, *** p < 0.001