Fig. 1

qRT-PCR analysis of LjASN genes expression in different tissues of L. japonicus . Plants were grown for 6 weeks and irrigated with Hornum medium containing NH₄NO₃ (a, c, e), or inoculated with M. loti and irrigated with Hornum medium without nitrogen (b, d, f). The samples were harvested at 4 h after the beginning of the light or dark period for the quantification of transcripts. Transcript levels are reported as relative units (r.u.). Data are the mean ± SE of three independent biological replicates