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Fig. 1 | BMC Genomics

Fig. 1

From: Integrative testis transcriptome analysis reveals differentially expressed miRNAs and their mRNA targets during early puberty in Atlantic salmon

Fig. 1

Description of samples and analysis procedure. (a) Gonadosomatic index (GSI, %; upper), 11-ketotestosterone levels (11KT, ng/ml; center) and the ratio of gene expression for igf3/amh (lower) in testis samples used for miRNA and mRNA-seq analysis. Statistical significant differences are marked with asterisk (*: p < =0.05, n.s.: non significant, according to Wilcoxon rank-sum statistic). (b) Representative histology (hematoxylin-eosin combined with periodic acid according to Schiff; HE/PAS; left column) and proliferation activity (immunocytochemical detection of phosphorylated histone H3; pH 3; right column, marked with triangles) in immature (Im), prepubertal (Pre) and pubertal (Pub) testis samples. Abbreviations: Au = type A undifferentiated spermatogonia, and Adiff = type A differentiating spermatogonia, SC = Sertoli Cells (c) Illustration of experimental setup and analysis pipeline used in this paper

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