Fig. 4

Manhattan plot of important QTL and haplotype analysis of candidate genes related to QTL including qSh6 (a), qSdw3a (b and c), qSdw3b (d and e), qSdw6 (f), qSdw11 (g), qRdw9 (h), qSwc5 (i), qSwc10 (j), qSFe2 (k) and qSZn11(l). Each point was a gene in the region of the QTL. Line and histogram in different colors indicated different conditions: green is control condition, yellow is ferrous iron stress condition, blue is zinc stress condition and red is the ratio of zinc stress to control condition. Dash line showed the threshold to determine candidate genes. The ** and *** suggested significance of ANOVA at p < 0.01and p < 0.001, respectively. The letter on histogram (a and b) indicated multiple comparisons result at the significant level 0.01. The value in bracket was the number of individuals for each haplotype