Fig. 2
From: Subfunctionalization influences the expansion of bacterial multidrug antibiotic resistance
Expression levels of RND 2- RND 4 genes in the B. cenocepacia J2315, D4, D4(pEP_RND2_operon), D4(pEP_RND4_operon), J2315(pEP_RND2_operon), and J2315(pEP_RND4_operon) strains. The quantity mean of RND 2-RND 4 mRNA /quantity mean of the 16S rRNA gene in the presence of 4 μg/ml of nalidixic acid in the B. cenocepacia J2315, D4, D4(pEP_RND2_operon), D4(pEP_RND4_operon), J2315(pEP_RND2_operon), and J2315(pEP_RND4_operon) strains are reported. The mean quantity of mRNA was estimated on the basis of the standard curve and for each target gene it was normalized on the mean quantity of 16S rRNA, used as a reference. The differences in genes expression was assessed using a t-test (* = P < 0.05)