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Table 1 Plasmids and strains used in this work

From: Subfunctionalization influences the expansion of bacterial multidrug antibiotic resistance

Strain or Plasmid

Description

Souce or reference

Plasmids

Vector

Insert cloned

Insert leight (bp)

Characteristic/genotype

 

 pGEM-T Easy

   

Vector for PCR cloning, Ampr

Promega

 pRK2013

   

ori colE1, RK2 derivative, Kanr, mob +, tra +

Figurski et al. 1979

 pSCrhaB2

   

ori pBBR1 rhaR rhaS P rhaB Tpr mob +

Cardona et al. 2005

 pEP_RND2_operon

pSCrhaB2

Operon RND_2 (promoter region,BCAS0766–64)

6178

Tpr

This work

 pEP_RND4_operon

pSCrhaB2

Operon RND_4 (promoter region, BCAL2822–20)

6288

Tpr

This work

Strains

Description

 

Accession number

  

 Escherichia coli

  DH5α

F− Φ80dlacZΔMI5 Δ(lacZYA-argF)U169 endA I recA I hsdRI7(rK −mk +) sup E44 thi-I ΔgyrA96 relA I

  

Laboratory stock, Bethesda Research Laboratories 1986

  SY327

araD Δ(lac pro) argE(Am) recA56 nalA λ pir; Rifr anche pRK2013

  

Miller et al. 1988

  SY327 (pRK2013, pEP_RND2_operon)

    

This work

  SY327 (pRK2013, pEP_RND4_operon)

    

This work

 Burkholderia cenocepacia

  J2315

CF clinical isolate

  

G. Manno, Buroni et al. 2009

  D4

J2315 ΔBCAL2820–BCAL2822

SRR3736982

 

Buroni et al. 2009

  J2315 (pEP_RND2_operon)

    

This work

  J2315 (pEP_RND4_operon)

    

This work

  D4 (pEP_RND2_operon)

    

This work

  D4 (pEP_RND4_operon)

    

This work

  D4/C_18

D4 derivative, obtained in the presence of Chloramphenicol

SRR3737008

 

This work

  D4/C_20

D4 derivative, obtained in the presence of Chloramphenicol

SRR3737019

 

This work