From: Subfunctionalization influences the expansion of bacterial multidrug antibiotic resistance
Strain or Plasmid | Description | Souce or reference | |||
---|---|---|---|---|---|
Plasmids | Vector | Insert cloned | Insert leight (bp) | Characteristic/genotype | Â |
 pGEM-T Easy |  |  |  | Vector for PCR cloning, Ampr | Promega |
 pRK2013 |  |  |  | ori colE1, RK2 derivative, Kanr, mob +, tra + | Figurski et al. 1979 |
 pSCrhaB2 |  |  |  | ori pBBR1 rhaR rhaS P rhaB Tpr mob + | Cardona et al. 2005 |
 pEP_RND2_operon | pSCrhaB2 | Operon RND_2 (promoter region,BCAS0766–64) | 6178 | Tpr | This work |
 pEP_RND4_operon | pSCrhaB2 | Operon RND_4 (promoter region, BCAL2822–20) | 6288 | Tpr | This work |
Strains | Description | Â | Accession number | Â | Â |
 Escherichia coli | |||||
  DH5α | F− Φ80dlacZΔMI5 Δ(lacZYA-argF)U169 endA I recA I hsdRI7(rK −mk +) sup E44 thi-I ΔgyrA96 relA I |  |  | Laboratory stock, Bethesda Research Laboratories 1986 | |
  SY327 | araD Δ(lac pro) argE(Am) recA56 nalA λ pir; Rifr anche pRK2013 |  |  | Miller et al. 1988 | |
  SY327 (pRK2013, pEP_RND2_operon) |  |  |  |  | This work |
  SY327 (pRK2013, pEP_RND4_operon) |  |  |  |  | This work |
 Burkholderia cenocepacia | |||||
  J2315 | CF clinical isolate |  |  | G. Manno, Buroni et al. 2009 | |
  D4 | J2315 ΔBCAL2820–BCAL2822 | SRR3736982 |  | Buroni et al. 2009 | |
  J2315 (pEP_RND2_operon) |  |  |  |  | This work |
  J2315 (pEP_RND4_operon) |  |  |  |  | This work |
  D4 (pEP_RND2_operon) |  |  |  |  | This work |
  D4 (pEP_RND4_operon) |  |  |  |  | This work |
  D4/C_18 | D4 derivative, obtained in the presence of Chloramphenicol | SRR3737008 |  | This work | |
  D4/C_20 | D4 derivative, obtained in the presence of Chloramphenicol | SRR3737019 |  | This work |