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Table 1 Plasmids and strains used in this work

From: Subfunctionalization influences the expansion of bacterial multidrug antibiotic resistance

Strain or Plasmid Description Souce or reference
Plasmids Vector Insert cloned Insert leight (bp) Characteristic/genotype  
 pGEM-T Easy     Vector for PCR cloning, Ampr Promega
 pRK2013     ori colE1, RK2 derivative, Kanr, mob +, tra + Figurski et al. 1979
 pSCrhaB2     ori pBBR1 rhaR rhaS P rhaB Tpr mob + Cardona et al. 2005
 pEP_RND2_operon pSCrhaB2 Operon RND_2 (promoter region,BCAS0766–64) 6178 Tpr This work
 pEP_RND4_operon pSCrhaB2 Operon RND_4 (promoter region, BCAL2822–20) 6288 Tpr This work
Strains Description   Accession number   
 Escherichia coli
  DH5α F Φ80dlacZΔMI5 Δ(lacZYA-argF)U169 endA I recA I hsdRI7(rK mk +) sup E44 thi-I ΔgyrA96 relA I    Laboratory stock, Bethesda Research Laboratories 1986
  SY327 araD Δ(lac pro) argE(Am) recA56 nalA λ pir; Rifr anche pRK2013    Miller et al. 1988
  SY327 (pRK2013, pEP_RND2_operon)      This work
  SY327 (pRK2013, pEP_RND4_operon)      This work
 Burkholderia cenocepacia
  J2315 CF clinical isolate    G. Manno, Buroni et al. 2009
  D4 J2315 ΔBCAL2820–BCAL2822 SRR3736982   Buroni et al. 2009
  J2315 (pEP_RND2_operon)      This work
  J2315 (pEP_RND4_operon)      This work
  D4 (pEP_RND2_operon)      This work
  D4 (pEP_RND4_operon)      This work
  D4/C_18 D4 derivative, obtained in the presence of Chloramphenicol SRR3737008   This work
  D4/C_20 D4 derivative, obtained in the presence of Chloramphenicol SRR3737019   This work