Fig. 6

Subgroup analysis of DNA methylation between LUSC and NTL. a Subgroup analysis of DNA methylation between LUSC and NTL according to smoking status, differentiation, TNM stage, age and complications. The correlation coefficient was given in the left corner. b Two-dimensional hierarchical clustering of all the significantly differently methylated probes in tumors was performed (n = 24). DNA methylation levels of Cluster 1, Custer 2 and NTL are shown using M-values. Note that methylation levels were significantly higher in Cluster 2 than in Cluster 1, although those of Cluster 1 were higher than those of NTL. c Two-dimensional hierarchical clustering of the 2470 differentially methylated probes (on the CpG island) was performed, two distinct clusters were identified. DNA methylation levels of Cluster 1, Custer 2 and NTL are shown using M-values. Note that methylation levels were significantly higher in Cluster 2 than in Cluster 1