Table 1 Mathematical analysis of the sgRNA off-target homologies
Total combination of 20Â bp | 1,099,511,627,776 |
|---|---|
Mismatches in seed sequence | Number of combinations |
0 | 1 |
1 | 36 |
2 | 594 |
Mismatches in non-seed sequence | Number of combinations |
0 | 1 |
1 | 24 |
2 | 252 |
3 | 1512 |
4 | 5670 |
Total base mismatches | 236,401 |
DNA bulge with 0 base mismatch | 64 |
RNA bulge with 0 base mismatch | 60 |
DNA bulge with 1 base mismatch | 2112 |
RNA bulge with 1 base mismatch | 1968 |
RNA bulge of two bases | 32 |
Total combinations of homologies | 240,637 |
Off-target homology probability of a 20-base DNA sequence | 0.00000021886 |
Probability of potential PAM | 0.2500 |
Off-target homology probability | 0.00000005471 |