Table 3 Comparison between CRISPOR, Cas-OFFinder and the proposed algorithm on off-target homology search for EMX1 sgRNA guide sequence
| Â | Number of off-target homologies identified | ||||
|---|---|---|---|---|---|
Number of base mismatches | 0 | 1 | 2 | 3 | 4 |
CRISPOR | 0 | 0 | 6 | 38 | 296 |
Cas-OFFinder | 0 | 0 | 1 | 18 | 273 |
Our Algorithm (with Secondary PAM) | 0 | 0 | 6 | 87 | 1227 |
Our Algorithm (without secondary PAM) | 0 | 0 | 1 | 18 | 273 |