Fig. 3
From: Optimisation of 16S rRNA gut microbiota profiling of extremely low birth weight infants
Comparison of taxonomic assignments among the 16S rRNA gene hypervariable regions tested using PE protocol approach. Heat map displaying number of reads assigned to the most common bacterial taxa found in the study samples. Top panel row divides the figure in the different regions of the 16 s rRNA gene analysed, namely: V1 + V2 + V3 (primers 27F-519R), V4 + V5 (primers 530F-926R) and V6 + V7 + V8 (primers 926F-1394R). The vertical axis of the panel indicates a selection of the 13 most common bacterial taxa found. The horizontal axis labels the different samples used in the study: preterms without supplementation (AP1E, AP5D, AP8C, AP25C), preterms with supplementation (P29F, P30N, P31B, P35C), and term baby samples (V2A, V3 J). The intensity of the green colour highlights the abundance of the number of reads found. Probiotic supplementation has been abbreviated to supplem. in the figure. Further information on the number of reads obtained for this study can be found in Additional file 19