Fig. 4
From: Optimisation of 16S rRNA gut microbiota profiling of extremely low birth weight infants
Principal Coordinate Analysis (PCoA) based on 16S rRNA community profiles analysed using PE protocol of the hypervariable regions tested. PCoA was performed based on the taxonomic assignments obtained from the 16S rRNA gene sequencing libraries analysed. Samples used for this plot were classified in main three groups: preterms without supplementation (AP1E, AP5D, AP8C, AP25C), preterms with supplementation (P29F, P30N, P31B, P35C), and term baby samples (V2A, V3Â J). Samples names are coded highlighting the 16S rRNA gene library they belong. Sample names ending in (.27F) belong to 16S rRNA gene library prepared using primers 27F-519R (target region V1Â +Â V2Â +Â V3), sample names ending in (.530F) belong to 16S rRNA gene library prepared using primers 530F-926R (region V4Â +Â V5), and sample names ending in (.926F) belong to 16S rRNA gene library amplified using primers 926F-1394R (region V6Â +Â V7Â +Â V8). PCoA plot indicates that distribution of samples targeting (V4Â +Â V5) region was distinct from samples targeting (V1Â +Â V2Â +Â V3) and (V6Â +Â V7Â +Â V8)