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Fig. 1 | BMC Genomics

Fig. 1

From: Development of amplicon deep sequencing markers and data analysis pipeline for genotyping multi-clonal malaria infections

Fig. 1

Mismatch rate per nucleotide position derived from all samples sequenced for markers cpmp and csp. Each data point represents the mean observed mismatch rate observed in all reads of one sample at the respective nucleotide position. Red data points: control samples (P. falciparum culture strains); black data points: field samples; X-axis: nucleotide position in sequenced fragment; Y-axis: mismatch rate with respect to the reference sequence (for control samples: sequences of strains 3D7 and HB3, for field samples, 3D7 sequence); dashed grey lines represent SNPs with a mismatch rate of >0.5 in >1 sample; red dotted horizontal line indicates a mismatch rate of 0.5; solid black vertical line: position of concatenation of forward and reverse reads

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BMC Genomics

ISSN: 1471-2164

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