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Table 5 Mean proportion of singleton or chimeric reads and indels detected in both field sample replicates

From: Development of amplicon deep sequencing markers and data analysis pipeline for genotyping multi-clonal malaria infections

Marker

Replicate 1

Replicate 2

 

Singletons

%

Indels

%

Chimera

%

Singletons

%

Indels

%

Chimera

%

csp

11.55

3.78a

0.00

11.47

4.05a

0.00

cpmp

9.76

0.073b

0.631c

9.74

0.034b

0.130c

  1. a Marker csp: Indels Replicate 1 versus 2; Student’s t-Test: t = −1.336, df = 71.052, p-value = 0.1858
  2. b Marker cpmp: Indels Replicate 1 versus 2; Student’s t-Test: t = 1.3304, df = 71.94, p-value = 0.1876
  3. c Marker cpmp: Chimera Replicate 1 versus 2; Student’s t-Test: t = 2.3552, df = 55.4, p-value = 0.02208
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BMC Genomics

ISSN: 1471-2164

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