Effect of short term diet restriction on gene expression in the bovine hypothalamus using next generation RNA sequencing technology

Table 3 Enriched genetic pathways between Restricted Anovulatory and Control groups from Innatedb

Pathway name	Genes	Total no. of genes in pathway	P value
RIG-I-like receptor signaling pathway	CXCL10 ↑, Q1KLR3 ↑, IFIH1 ↑, IL8 ↑	70	0.000815541
Activation of NMDA receptor upon glutamate binding and postsynaptic events	ADCY1 ↓, GRIN2A ↓, GRIN2C ↓	32	0.002998833
Post NMDA receptor activation events	ADCY1 ↓, GRIN2A ↓, GRIN2C ↓	32	0.002998833
Long-term potentiation	ADCY1 ↓, GRIN2A ↓, GRIN2C ↓, ITPR1 ↓	68	0.003241851
Cytosolic DNA-sensing pathway	CXL10 ↑, Q1KLR3 ↑, ZBP1 ↑	55	0.003482075
Transmission across Chemical Synapses	ADCY1 ↓, GRIN2A ↓, GRIN2C ↓, SLC6A3 ↓	76	0.00456686
Synaptic Transmission	ADCY1 ↓, GRIN2A ↓, GRIN2C ↓, SLC6A3 ↓	81	0.005631178
Gata3 participate in activating the th2 cytokine genes expression	ADCY1 ↓, IL1A ↑	18	0.007137619
D-Arginine and D-ornithine metabolism	DAO ↓	1	0.007150141
Neuroactive ligand-receptor interaction	CALRL ↑, A6QQP6 ↑, GRIK3 ↓, GRIN2A ↓, GRIN2C ↓, TRY2 ↑	301	0.009258012
Neuroransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell	ADCY1 ↓, GRIN2A ↓, GRIN2C ↓	48	0.009603978
Unblocking of NMDA receptor, glutamate binding and activation	GRIN2A ↓, GRIN2C ↓	12	0.009663882

Expression is Restricted Anovulatory relative to Control. eg. CXL10 ↑ means RA has increased expression compared to C

ISSN: 1471-2164