Fig. 5
From: Transcriptome sequencing and analysis of zinc-uptake-related genes in Trichophyton mentagrophytes
PCR analysis of transformants. a Amplification of hph (1200Â bp) using the primers hph-F and hph-R. Lane 1, DNA sample from the T. mentagrophytes ZafA gene mutant strain; lane 2, DNA sample from the wild-type T. mentagrophytes strain 28,185, lane 3, the transformation vector pDHt/ZafA:: hph. b Amplification of the ZafA gene fragment disrupted (400Â bp) using the primers ZafA-F and ZafA-R. Lane 1, DNA sample from the T. mentagrophytes ZafA gene mutant strain; lane 2, the transformation vector pDHt/ZafA:: hph; lane 3, DNA sample from the wild-type T. mentagrophytes strain 28,185