Fig. 1
From: ENCoRE: an efficient software for CRISPR screens identifies new players in extrinsic apoptosis
An overview of a typical CRISPR screen. Lentiviral particles containing pooled guide sequences are added to cells expressing Cas9 nuclease. Following selection and amplification, guides are deep sequenced from genomic DNA, filtered, and correlated to genes or chromosomal position. Statistical analysis and pathway deconvolution complete the initial assessment after which candidates are individually validated